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3.5.1.119: Pup amidohydrolase

This is an abbreviated version!
For detailed information about Pup amidohydrolase, go to the full flat file.

Word Map on EC 3.5.1.119

Reaction

[prokaryotic ubiquitin-like protein]-L-glutamine
+
H2O
=
[prokaryotic ubiquitin-like protein]-L-glutamate
 +
NH3

Synonyms

deamidase of Pup, deamidase/depupylase, deamidase/depupylase Dop, depupylase, depupylase Dop, depupylase/deamidase, Dop, DPUP, Pup deamidase, Rv2112, Rv2112c

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.119 Pup amidohydrolase

Application

Application on EC 3.5.1.119 - Pup amidohydrolase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
drug development
-
the Pup-proteasome enzymes are essential for full virulence and persistence in the mammalian host. As such, the Pup-proteasome enzymes are potential targets for development of antituberculosis therapeutics. Such development requires sensitive and robust assays for measurements of enzymatic activities and the effect of examined inhibitors. An in vitro activity assay for Dop, the first enzyme in the Pup-proteasome system is described, that is ased on fluorescence anisotropy measurements. This assay is simple, sensitive, and compatible with a high-throughput format for screening purposes and can be reliably and conveniently used for detailed kinetic measurements of Dop activity
medicine
-
since Dop is necessary for persistent infection by Mycobacterium tuberculosis, its selective inhibition holds potential for tuberculosis therapy
pharmacology