3.4.24.B23: stage IV sporulation protein FB
This is an abbreviated version!
For detailed information about stage IV sporulation protein FB, go to the full flat file.
Reaction
implicated in the coupling of mother cell to forespore gene expression. Required for spore formation. Processes the pro-sigma K factor =
Synonyms
More, pro-sigmaK protease, SPOIVFB, SpoIVFB protein
ECTree
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Purification
Purification on EC 3.4.24.B23 - stage IV sporulation protein FB
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when catalytically inactive SpoIVFB is coexpressed with C-terminally truncated Pro-sigmaK(1-126) in Escherichia coli, the substrate dramatically improves solubilization of the enzyme from membranes with mild detergents. Both the Pro(1-20) and sigmaK(21-126) parts contribute to improving SpoIVFB solubilization from membranes, but only the sigmaK part is needed to form a stable complex with SpoIVFB. The last 10 residues of SpoIVFB are required for improved solubilization from membranes