3.4.24.B23: stage IV sporulation protein FB
This is an abbreviated version!
For detailed information about stage IV sporulation protein FB, go to the full flat file.
Reaction
implicated in the coupling of mother cell to forespore gene expression. Required for spore formation. Processes the pro-sigma K factor =
Synonyms
More, pro-sigmaK protease, SPOIVFB, SpoIVFB protein
ECTree
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Engineering
Engineering on EC 3.4.24.B23 - stage IV sporulation protein FB
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E44Q
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site-directed mutagenesis of SpoIVFB protein with an extra transmembrane segment , the mutant is inactive with substrate mutant pro-sigmaK S20G in contrast to the wild-type enzyme
additional information
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construction of SpoIVFB protein with an extra transmembrane segment attached to the N-terminus for increased expression rate and facilitated purification, overview. Deletion of 10 residues from the C-terminal end of SpoIVFB nearly eliminates cleavage of coexpressed pro-sigmaK in recombinant Escherichia coli