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3.4.24.81: ADAM10 endopeptidase

This is an abbreviated version!
For detailed information about ADAM10 endopeptidase, go to the full flat file.

Word Map on EC 3.4.24.81

Reaction

endopeptidase of broad specificity =

Synonyms

a disintegrin and metalloprotease 10, a disintegrin and metalloproteinase 10, a disintegrin and metalloproteinase-10, a-disintegrin-and-metalloprotease 10, AD10, ADAM 10, ADAM-10, ADAM10, CD156c, CD23 metalloprotease, HsT18717, kuz, kuzbanian, Kuzbanian protein, MADM, mammalian disintegrin-metalloprotease, metalloproteinase 10, metalloproteinase ADAM10, metalloproteinase Kuzbanian, metalloproteinase MADM, metalloproteinase-disintegrin, myelin-associated disintegrin metalloproteinase, notch proteinase, transmembrane metzinkin-protease of the a disintegrin and metalloproteinase family-10

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.81 ADAM10 endopeptidase

Cloned

Cloned on EC 3.4.24.81 - ADAM10 endopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a C-terminal HA-tagged fusion protein is transfected in human ovarian cancer SKOV3 cells and human embryonic kidney HEK293 cells
ADAM10 is expressed as a wild-type protein and as a FLAG-tagged ADAM10 fusion protein in which the tag is inserted immediately C-terminal to the proprotein convertase recognition sequence RKKR. Expression is carried out by stable transfection of a human neuroblastoma SH-SY5Y cell line. Internally tagged ADAM10 fusion protein is easily detected in Western blot analysis. Catalytic activity of wild-type and internally tagged protein is indistinguishable
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expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3)Star cells
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expressed in HEK-293 cells
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expressed in human embryonic kidney HEK293 cell line
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expressed in HUVEC and COS-7 cells
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expressed in Ls174T human colon cancer cells
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expressed in MEF cells and 293-T cells
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expressed in U-251 cells
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expression in CHO cells
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generation of ADAM10—chimeras of the extracellular domain of the human interleukin-2 receptor with the intracellular C-terminal domain of mouse ADAM10. Transfection into COS7 cells
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produced in insect cells by baculoviral expression as truncated constructs consisting of the signal sequence, pro- and catalytic domains fused to a C-terminal His6 (hexahistidine) tag (residues 1–460 of mouse ADAM10).
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