Cloned (Comment) | Organism |
---|---|
ADAM10 is expressed as a wild-type protein and as a FLAG-tagged ADAM10 fusion protein in which the tag is inserted immediately C-terminal to the proprotein convertase recognition sequence RKKR. Expression is carried out by stable transfection of a human neuroblastoma SH-SY5Y cell line. Internally tagged ADAM10 fusion protein is easily detected in Western blot analysis. Catalytic activity of wild-type and internally tagged protein is indistinguishable | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA672 | a truncated soluble construct of ADAM10 lacking the transmembrane and cytosolic domains (truncation after Glu680), although correctly post-translationally processed and catalytically active with respect to a synthetic peptide substrate, is incapable of shedding cell-associated amyloid precursor protein (APP) | Homo sapiens |
additional information | a GPI-anchored form of ADAM10 lacking the cytosolic, transmembrane and a-helical juxtamembrane regions of the wild-type protein is shed in a similar manner. Mutant fusion construct consists of the first 652 residues of wild-type ADAM10 fused, via a two residue linker, to the 24 residue GPI anchor signal sequence of human carboxypeptidase M | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GW4023 | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE, mature form wild-type | Homo sapiens |
98000 | - |
SDS-PAGE, immature form wild-type | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | ADAM10 itself is subject to ectodomain shedding via a mechanism which is inhibited by ADAM inhibitor (GW4023) and stimulated by phorbol ester treatment of cells. The treatment of cells with GW4023 causes a reciprocal accumulation of membrane-associated mature ADAM10 in both cell lysates and extracellular membrane vesicles. ADAM9 is, at least in part, responsible for the ectodomain shedding of ADAM10 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic activity of wild-type and internally FLAG-tagged fusion protein is indistinguishable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid precursor protein + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAM10 | - |
Homo sapiens |