3.4.24.12: envelysin

This is an abbreviated version!
For detailed information about envelysin, go to the full flat file.

Reaction

Hydrolysis of proteins of the fertilization envelope and dimethylcasein =

Synonyms

chorion-digesting proteinase, chorionase, chymostrypsin, Envelysin, hatching enzyme, HCE, HE, HEz, High choriolytic enzyme, LCE, Low choriolytic enzyme, proteinase, chorion-digesting, sea urchin embryo hatching enzyme, sea-urchin-hatching proteinase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.12 envelysin

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Results	in table
15	AA Sequence
2	Application
1	CAS Registry Number
2	Cloned(Commentary)
8	General Stability
39	Inhibitors
4	KM Value [mM]
2	Localization
11	Metals/Ions
19	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
18	Organism
2	pH Optimum
1	pH Range
2	pH Stability
2	Posttranslational Modification
8	Purification (Commentary)
1	Reaction
2	Reaction Type
18	Reference
13	Source Tissue
4	Specific Activity [micromol/min/mg]
2	Storage Stability
29	Substrates and Products (Substrate)
10	Subunits
19	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Molecular Weight

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of the Enzyme Summary Page.

Molecular Weight on EC 3.4.24.12 - envelysin

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

135000

Hemicentrotus pulcherrimus

-

hatching enzyme secreted from the blastula with the fertilization envelope, gel filtration

36978

150000

Strongylocentrotus purpuratus

-

gel filtration

36999

22500

Strongylocentrotus purpuratus

-

1 * 22500, fertilized and unfertilized eggs

36985

23000

Strongylocentrotus purpuratus

-

gel filtration

36985

28500

Strongylocentrotus purpuratus

-

1 * 28500, SDS-PAGE

36983, 36984

30000

2 entries

33000

Strongylocentrotus purpuratus

-

x * 33000, the enzyme is initially secreted as a 57000 Da protein, during purification it is converted to the 33000 Da form, SDS-PAGE

36996

44000

Strongylocentrotus intermedius

-

44000, SDS-PAGE

36982

45000

Strongylocentrotus intermedius

-

gel filtration

36982

47700

Echinoidea

-

-

668835

51000

Paracentrotus lividus

-

1 * 51000, SDS-PAGE

36974

57000

Paracentrotus lividus

-

gel filtration

36974

65000

Paracentrotus lividus

-

x * 65000, calculation from nucleotide sequence

37000

66123

Hemicentrotus pulcherrimus

P91953

x * 66123, calculation from nucleotide sequence

36993

80000

Hemicentrotus pulcherrimus

-

hatching enzyme secreted from the blastula without the fertilization envelope, gel filtration

36978

89000

Hemicentrotus pulcherrimus

-

intracellular hatching enzyme

36978

additional information

Hemicentrotus pulcherrimus

-

-

36978

30000

Strongylocentrotus purpuratus

-

-

36984

30000

Strongylocentrotus purpuratus

-

gel filtration

36983