Information on EC 3.4.24.12 - envelysin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.24.12
-
RECOMMENDED NAME
GeneOntology No.
envelysin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins of the fertilization envelope and dimethylcasein
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
endopeptidase; peptides, endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
50812-13-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr + H2O
Ala-Ser-Thr-Thr-Thr-Asn + Tyr-Thr
show the reaction diagram
-
i.e. peptide T, partial cleavage at Asn6-Tyr7
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
Arg-Pro-Lys-Pro-Gln-Gln + Phe-Phe-Gly-Leu-Met-NH2
show the reaction diagram
-
i.e. substance P
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg
show the reaction diagram
-
i.e. bradykinin, partial cleavage at Pro7-Phe8
-
?
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
-
i.e. angiotensin I, partial cleavage at Pro7-Phe8
-
?
benzyloxycarbonyl-Glu-Phe + H2O
benzyloxycarbonyl-Glu + Phe
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Glu-Tyr + H2O
Benzyloxycarbonyl-Glu + Tyr
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
Dimethylcasein + H2O
?
show the reaction diagram
egg envelope
?
show the reaction diagram
-
proteolyzes the fertilization envelope, thereby allowing the embryo to hatch
-
-
-
fertilization envelope + H2O
?
show the reaction diagram
Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2 + H2O
?
show the reaction diagram
-
i.e. mastoparan, clevage sites: Asn2-Leu3, 100%, Ala7-Ala8, 100%, Ala10-Lys11, 47%, and Lys12-Ile13, 47%
-
-
?
insulin
?
show the reaction diagram
-
oxidized B chain of insulin cleaved at FVNQ-HLCGSHLVEA-LY-LVCGERGFF-YTPKA
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-Tyr + ethanol
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
-
physalaemin + H2O
?
show the reaction diagram
-
-
-
-
?
snake venom alpha-protease
?
show the reaction diagram
-
poor substrate
-
-
?
thermolysin + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys + H2O
Tyr-Gly-Gly-Phe + Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys
show the reaction diagram
-
i.e. dynorphin A(1-13), partial cleavage at Phe4-Leu5
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro + H2O
Tyr-Gly-Gly-Phe + Leu-Arg-Lys-Tyr-Pro
show the reaction diagram
-
i.e. beta-neoendorphin, partial cleavage at Phe4-Leu5
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
egg envelope
?
show the reaction diagram
-
proteolyzes the fertilization envelope, thereby allowing the embryo to hatch
-
-
-
fertilization envelope + H2O
?
show the reaction diagram
Sea urchin
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NH4Cl
-
order of activation among monovalent cations: NaCl, KCl, NH4Cl
additional information
-
a moderate halophilic enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-Nitro-4-carboxyphenyl N,N-diphenylcarbamate
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degradation of N-benzoyl-L-tyrosine ethyl ester
Ac-Pro-Arg-Cys-Gly-Val-Pro-Asp-NH2
Sea urchin
-
-
acetyl-Pro-Arg-Cys-Gly-Val-Pro-Asp-Val-NH2
-
-
alpha2-Macroglobulin
-
-
-
chymostatin
diisopropyl phosphofluoridate
-
-
jaspisin
Sea urchin
-
-
N-tosyl-L-phenylalanine chloromethyl ketone
-
degradation of casein
p-nitrophenyl p'-guanidinobenzoate
-
-
Pepstatin
-
slight
phenylmethylsulfonyl fluoride
-
-
reactive red 120
-
-
Soybean trypsin inhibitor
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.84
dimethylated casein
-
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038 - 180
dimethylated casein
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
-
pH 6.5: about 55% of maximal activity, pH 9.0: about 45% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26 - 50
-
26°C: about 50% of maximal activity, 50°C: about 35% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
unfertilized and fertilized
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
Sea urchin
-
envelysin is probably packaged in secretory vesicles first localized between nucleus and the apical face
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22500
-
1 * 22500, fertilized and unfertilized eggs
23000
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gel filtration
28500
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1 * 28500, SDS-PAGE
33000
-
x * 33000, the enzyme is initially secreted as a 57000 Da protein, during purification it is converted to the 33000 Da form, SDS-PAGE
44000
-
44000, SDS-PAGE
45000
-
gel filtration
47700
Sea urchin
-
-
51000
-
1 * 51000, SDS-PAGE
57000
-
gel filtration
65000
-
x * 65000, calculation from nucleotide sequence
66123
x * 66123, calculation from nucleotide sequence
80000
-
hatching enzyme secreted from the blastula without the fertilization envelope, gel filtration
89000
-
intracellular hatching enzyme
135000
-
hatching enzyme secreted from the blastula with the fertilization envelope, gel filtration
150000
-
gel filtration
additional information
-
-
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no modification
-
contains no carbohydrate
side-chain modification
-
glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
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5 min, more than 95% loss of activity
36984
5 - 9
-
0°C, stable between
36982
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
CaCl2 and KCl or NaCl is required for stability
-
glycerol, 10% v/v, improves stability to either freezing or heating
-
glycerol, 5% v/v, protects from inactivation
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low concentrations of albumin considerably stabilize the enzyme
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stable to freezing and thawing
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the enzyme is resistant to urea, freezing-thawing and lyophilization
-
the enzyme is stabilized by CaCl2, NaCl and KCl. The enzyme is stabilized by dialysis against 1 M NaCl or KCl in the presence of CaCl2
-
the mature 50000 Da enzyme is highly susceptible to autolytic cleavage at Gln503-Leu504, producing the 38000 Da form retaining catalytic activity and substrate specificity against fertilization envelope
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, undilute, stable for weeks
-
-80°C, undilute, stable for months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by hydrophobic chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
Sea urchin
-
one of the earliest zygotic genes activated, about 12-20 h after fertilization, the hatching enzyme is secreted, digesting the fertilization envelope and freeing the swimming blastula
molecular biology
-
Ets4 is one of the transcription factors that control HE expression