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Literature summary for 3.4.24.12 extracted from

  • Gache, C.; Lepage, T.; Croce, J.; Lhomond, G.
    Envelysin (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 575-578.
No PubMed abstract available

Application

Application Comment Organism
degradation one of the earliest zygotic genes activated, about 12-20 h after fertilization, the hatching enzyme is secreted, digesting the fertilization envelope and freeing the swimming blastula Echinoidea
molecular biology Ets4 is one of the transcription factors that control HE expression Strongylocentrotus purpuratus

Cloned(Commentary)

Cloned (Comment) Organism
-
Paracentrotus lividus

Inhibitors

Inhibitors Comment Organism Structure
Ac-Pro-Arg-Cys-Gly-Val-Pro-Asp-NH2
-
Echinoidea
EDTA completely inhibited by 20 mM, inhibition is not reversible by Ca2+ Echinoidea
jaspisin
-
Echinoidea
Li+ shifts the border of the envelysin expression domain towards the animal pole Echinoidea
TIMP-1
-
Hemicentrotus pulcherrimus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information envelysin is probably packaged in secretory vesicles first localized between nucleus and the apical face Echinoidea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for full activity Echinoidea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47700
-
-
Echinoidea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fertilization envelope + H2O Echinoidea
-
?
-
?

Organism

Organism UniProt Comment Textmining
Echinoidea
-
-
-
Hemicentrotus pulcherrimus
-
-
-
Paracentrotus lividus
-
-
-
Strongylocentrotus purpuratus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by hydrophobic chromatography Paracentrotus lividus
by hydrophobic chromatography Strongylocentrotus purpuratus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Echinoidea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fertilization envelope + H2O
-
Echinoidea ?
-
?
insulin oxidized B chain of insulin cleaved at FVNQ-HLCGSHLVEA-LY-LVCGERGFF-YTPKA Hemicentrotus pulcherrimus ?
-
?
additional information short proteolyzed envelysin lacking the C-terminal part preferentially cleaves peptide bonds on the amino side of hydrophobic bulky residues (-Leu,-Ile,-Phe,-Tyr) Hemicentrotus pulcherrimus ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Echinoidea

Synonyms

Synonyms Comment Organism
hatching enzyme
-
Hemicentrotus pulcherrimus
hatching enzyme
-
Echinoidea
hatching enzyme
-
Paracentrotus lividus
hatching enzyme
-
Strongylocentrotus purpuratus
HE
-
Strongylocentrotus purpuratus