3.4.22.71: sortase B
This is an abbreviated version!
For detailed information about sortase B, go to the full flat file.
Word Map on EC 3.4.22.71
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3.4.22.71
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aureus
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peptidoglycan
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anthracis
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envelope
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transpeptidases
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cross-bridges
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isdc
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pentaglycine
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pilin
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transpeptidation
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analysis
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medicine
- 3.4.22.71
- aureus
- peptidoglycan
- anthracis
- envelope
- transpeptidases
-
cross-bridges
- isdc
- pentaglycine
- pilin
-
transpeptidation
- analysis
- medicine
Reaction
The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a NPXTN motif is cleaved. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =
Synonyms
CD630_27180, class B sortase, CPE0513, sortase B, Spy0129, SrtB, StrB
ECTree
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Purification
Purification on EC 3.4.22.71 - sortase B
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Ni-charged HiTrap column chromatography and Superdex 200 gel filtration
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recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) pLysS by affinity chromatography