3.4.22.71: sortase B
This is an abbreviated version!
For detailed information about sortase B, go to the full flat file.
Word Map on EC 3.4.22.71
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3.4.22.71
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aureus
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peptidoglycan
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anthracis
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envelope
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transpeptidases
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cross-bridges
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isdc
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pentaglycine
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pilin
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transpeptidation
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analysis
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medicine
- 3.4.22.71
- aureus
- peptidoglycan
- anthracis
- envelope
- transpeptidases
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cross-bridges
- isdc
- pentaglycine
- pilin
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transpeptidation
- analysis
- medicine
Reaction
The enzyme catalyses a cell wall sorting reaction in which a surface protein with a sorting signal containing a NPXTN motif is cleaved. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan. =
Synonyms
CD630_27180, class B sortase, CPE0513, sortase B, Spy0129, SrtB, StrB
ECTree
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Substrates Products
Substrates Products on EC 3.4.22.71 - sortase B
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REACTION DIAGRAM
AbpA-LPKTSAVKLE-green fluorescent protein + H2O
AbpA-LPKT + SAVKLE-green fluorescent protein
Lmo2186 + H2O
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NPKSS is a sorting motif of Lmo2186. Recognition of NPKSS by SrtB, even when placed in the context of the heterologous sorting signal of Lmo2185. Proline at position 2, and not lysine at position 3, is essential for the recognition of NPKSS by SrtB
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SvpA + H2O
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anchoring of SvpA to the bacterial cell wall is specifically mediated by SrzB
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(amylase-binding adhesin AbpA)2 + H2O
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2 amylase-binding adhesin AbpA
(amylase-binding adhesin AbpA)2 + H2O
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AbpA-LPKT + SAVKLE-green fluorescent protein
C-terminal sequence of AbpA fused to green fluorescent protein
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AbpA-LPKTSAVKLE-green fluorescent protein + H2O
AbpA-LPKT + SAVKLE-green fluorescent protein
C-terminal sequence of AbpA fused to green fluorescent protein
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Dabcyl-QANPQTNEE-Edans + H2O
Dabcyl-QANPQT + NEE-Edans
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IsdC is a surface protein necessary for heme iron uptake in Bacillus anthracis. SrtB recognizes the NPKTG sequence present
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IsdC + H2O
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the enzyme cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and rethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges
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IsdC + H2O
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the enzyme anchors the IsdC precursor with a C-terminal NPQTN motif sorting sognal, to the cell wall envelope. The sorting signal of IsdC is cleaved between threonine and asparagine of the NPQTN motif, and the carboxyl group of the thrteonine is amide-linked to the amino group of pentaglycine cross-bridges
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sortase B may be critical in the early stage of inhaltation anthrax
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additional information
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surface protein IsdC and sortase B are required for heme-iron scavenging of Bacillus anthracis
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additional information
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anchoring of SvpA to the bacterial cell wall is specifically mediated by SrzB. The enzyme is involved in the attachment of a subset of proteins to the cell wall, most likely by recognizing an NXZTN sorting motif. SrtB-mediated anchoring can be required to anchor surface proteins involved in the adaption of this microorganism to different environmental conditions
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additional information
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the svpA-srtB locus is regulated by iron availability, mediated by Fur
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additional information
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non-gel proteomics is a powerful technique to rapidly identify sortase substrates and to gain insights on potential sorting motifs. Two surface proteins, Lmo2185 and Lmo2186 are identified only when SrtB is active. The analysis of the peptides identified in these proteins suggests that SrtB of Listeria monocytogenes may recognize two different sorting motifs, NXZTN and NPKXZ
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additional information
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gram-positive pathogenic bacteria display proteins on their surface that play important roles during infection. In Staphylococcus aureus these surface proteins are anchored to the cell wall by two sortases, sortase A and sortaseB that recognize specific surface protein sorting signals. Sortase B plays a contributing role during the pathogenesis of staphylococcal infections
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