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3.2.1.99: arabinan endo-1,5-alpha-L-arabinanase

This is an abbreviated version!
For detailed information about arabinan endo-1,5-alpha-L-arabinanase, go to the full flat file.

Word Map on EC 3.2.1.99

Reaction

alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
+
H2O
=
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
 +
alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose

Synonyms

1,5-alpha-arabinanase A, ABN, ABN A, ABN-TS, Abn1, Abn2, ABNA, abnA1, abnA2, ABNase, Abnc, AbnS1, AbnZ1, alpha-1,5-L-endo-arabinanase, alpha-L-arabinohydrolase, ARA, ARA1, araban 5-alpha-L-arabinohydrolase, arabinanase A, arabinase, endo-1,5-alpha-L-, arase, Arb43A, Arb43B, arbA, BlAbn1, CEDAase, cold-adapted endo-arabinanase, endo-(1,5)-alpha-L-arabinanase, endo-(1->5)-alpha-L-arabinanase, endo-1,5-alpha-arabinanase, endo-1,5-alpha-L-arabinanase, endo-1,5-alpha-L-arabinase, endo-1,5-alpha-L-arabinase A, endo-1,5-arabinanase, endo-1,5-arabinase, endo-ABA, endo-alpha-(1->5)-L-arabinanase, endo-alpha-1,5-arabanase, endo-alpha-1,5-L-arabinanase, endo-alpha-arabinanase, endo-ara, endo-arabanase, endo-arabinanase, endo-arabinase, endo-D-arabinase, endo-L-arabinanase, endoarabinanase, endoarabinase, GH43 arabinanase, GH43 endo-arabinanase, More, PPase-C, protopectinase C, protopectinase-C, Tpet_0637

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.99 arabinan endo-1,5-alpha-L-arabinanase

Cloned

Cloned on EC 3.2.1.99 - arabinan endo-1,5-alpha-L-arabinanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Abn2 overexpressed in Escherichia coli BL21(DE3) pLysS harboring pZI39
cDNA clone, overexpression in Aspergillus niger and Aspergillus nidulans
-
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
expressed in Bacillus subtilis
-
expressed in Bacillus subtilis strain MI112
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli ER2566 cells
-
expressed in Nicotiana benthamiana
-
expressed in Pichia pastoris strain GS115
expression in Escherichia coli
expression in Escherichia coli BL21(DE3) cells as fusion proteins with a His-tag at the C-terminus
expression in Pichia pastoris
gene abnB, expression of wild-type and mutant enzymes
hyperexpression in Escherichia coli
-
overexpressed in Escherichia coli Top10
overexpression of His-tagged enzyme in Escherichia coli
-
wild-type ligated into vector pET30a(+) and overexpressed in Escherichia coli BL21 (DE3) pLysS harbouring pZI39. Selenol-methionine-labelled protein overexpressed in an auxotrophic Escherichia coli strain B834 (DE3) harbouring pZI39