Literature summary for 3.2.1.99 extracted from

de Sanctis, D.; Bento, I.; Inacio, J.M.; Custodio, S.; de Sa-Nogueira, I.; Carrondo, M.A.
Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis (2008), Acta Crystallogr. Sect. F, 64, 636-638.

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type ligated into vector pET30a(+) and overexpressed in Escherichia coli BL21 (DE3) pLysS harbouring pZI39. Selenol-methionine-labelled protein overexpressed in an auxotrophic Escherichia coli strain B834 (DE3) harbouring pZI39	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
native and seleno-methionine-labelled derivative, by sitting-drop vapour-diffusion method, to 1.9 and 2.7 A resolution, respectively. Native protein crystals appear in two different space groups, P1, with unit-cell parameters a=51.9, b=57.6, c=86.2 A, alpha=82.3, beta=87.9, gamma=63.6, and P212121, with unit-cell parameters a=57.9, b=163.3, c=202.0 A. The selenol-methionine-labelled derivative of Abn2 only crystallizes in one crystal form, which is similar to the form of space group P212121 of the wild-type protein, with unit-cell parameters a=57.84, b=163.22, c=201.85 A, which can accommodate four molecules (each containing 13 selenomethionine residues) in the asymmetric unit	Bacillus subtilis

Crystallization (Comment)

Organism

native and seleno-methionine-labelled derivative, by sitting-drop vapour-diffusion method, to 1.9 and 2.7 A resolution, respectively. Native protein crystals appear in two different space groups, P1, with unit-cell parameters a=51.9, b=57.6, c=86.2 A, alpha=82.3, beta=87.9, gamma=63.6, and P212121, with unit-cell parameters a=57.9, b=163.3, c=202.0 A. The selenol-methionine-labelled derivative of Abn2 only crystallizes in one crystal form, which is similar to the form of space group P212121 of the wild-type protein, with unit-cell parameters a=57.84, b=163.22, c=201.85 A, which can accommodate four molecules (each containing 13 selenomethionine residues) in the asymmetric unit

Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P42293	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and selenol-methionine-labelled protein purified on nickel column, more than 95% pure	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
Abn2	-	Bacillus subtilis
endo-1,5-alpha-arabinanase	-	Bacillus subtilis

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Release 2023.1 (January 2023)