3.2.1.99: arabinan endo-1,5-alpha-L-arabinanase
This is an abbreviated version!
For detailed information about arabinan endo-1,5-alpha-L-arabinanase, go to the full flat file.
Word Map on EC 3.2.1.99
-
3.2.1.99
-
arabinofuranosidase
-
alpha-l-arabinofuranosidase
-
debranched
-
arabinases
-
arabinotriose
-
arabino-oligosaccharides
-
nutrition
-
magnetospheric
-
endo-galactanase
-
l-arabitol
-
analysis
-
rhamnogalacturonans
-
lipoarabinomannans
-
arabinose-containing
-
exo-acting
-
thermodenitrificans
-
five-bladed
-
p-nitrophenyl-alpha-l-arabinofuranoside
-
cellvibrio
-
biofuel production
-
degradation
-
synthesis
-
industry
- 3.2.1.99
- arabinofuranosidase
- alpha-l-arabinofuranosidase
-
debranched
-
arabinases
- arabinotriose
- arabino-oligosaccharides
- nutrition
-
magnetospheric
-
endo-galactanase
- l-arabitol
- analysis
- rhamnogalacturonans
- lipoarabinomannans
-
arabinose-containing
-
exo-acting
- thermodenitrificans
-
five-bladed
- p-nitrophenyl-alpha-l-arabinofuranoside
- cellvibrio
- biofuel production
- degradation
- synthesis
- industry
Reaction
Synonyms
1,5-alpha-arabinanase A, ABN, ABN A, ABN-TS, Abn1, Abn2, ABNA, abnA1, abnA2, ABNase, Abnc, AbnS1, AbnZ1, alpha-1,5-L-endo-arabinanase, alpha-L-arabinohydrolase, ARA, ARA1, araban 5-alpha-L-arabinohydrolase, arabinanase A, arabinase, endo-1,5-alpha-L-, arase, Arb43A, Arb43B, arbA, BlAbn1, CEDAase, cold-adapted endo-arabinanase, endo-(1,5)-alpha-L-arabinanase, endo-(1->5)-alpha-L-arabinanase, endo-1,5-alpha-arabinanase, endo-1,5-alpha-L-arabinanase, endo-1,5-alpha-L-arabinase, endo-1,5-alpha-L-arabinase A, endo-1,5-arabinanase, endo-1,5-arabinase, endo-ABA, endo-alpha-(1->5)-L-arabinanase, endo-alpha-1,5-arabanase, endo-alpha-1,5-L-arabinanase, endo-alpha-arabinanase, endo-ara, endo-arabanase, endo-arabinanase, endo-arabinase, endo-D-arabinase, endo-L-arabinanase, endoarabinanase, endoarabinase, GH43 arabinanase, GH43 endo-arabinanase, More, PPase-C, protopectinase C, protopectinase-C, Tpet_0637
ECTree
3.2.1.99 arabinan endo-1,5-alpha-L-arabinanaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.99 - arabinan endo-1,5-alpha-L-arabinanase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1->5)-L-alphaarabinan + H2O
(1->5)-L-arabinooligosaccharides
-
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP6
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinobiose + L-arabinotetraose and L-arabinotriose
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP7
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinotriose + L-arabinotetraose
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
branched arabinan + H2O
?
-
branched arabinan from sugar beet. Hydrolyzed at 12% compared to debranched arabinan
-
-
?
cell wall polysaccharide + H2O
arabinose + galactose + rhamnose + fucose
-
F-11, apple
F-11, 34.3% arabinose + 10% galactose + 2.6% rhamnose/fucose
?
debranched (1->5)-L-arabinan + H2O
L-arabinose
-
under the optimum conditions, 16 g/l L-arabinose is obtained from 20 g/l debranched arabinan with a hydrolysis yield of 80%
-
-
?
debranched (1->5)-L-arabinan + H2O
L-arabinose + L-arabinosyl-alpha-(1->5)-L-arabinoside + ?
-
-
-
?
debranched arabinan + H2O
arabinose + arabinobiose + arabinotriose
-
-
-
?
reduced arabinohexaose + H2O
arabinotriose + reduced arabinotriose
-
the enzyme hydrolyzes a branched arabinohexaose with a double substituted arabinose at subsite-2
-
-
?
sugar beet arabinan + H2O
L-arabinose + L-arabinosyl-alpha-(1->5)-L-arabinoside
48% of the activity with debranched (1->5)-L-arabinan
-
-
?
sugar beet arabinan arabinan + H2O
L-arabinose + ?
-
-
conversion yield of 83% at pH 5.0 and 75°C for 216 h
-
?
1,5-alpha-L-arabinan + H2O
?
Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters
-
-
?
1,5-alpha-L-arabinan + H2O
?
Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters
-
-
?
1,5-alpha-L-arabinan + H2O
?
debranched arabinan, best substrate
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + ?
-
linear arabinan
main product
?
1,5-alpha-L-arabinan + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + ?
-
linear arabinan
main product
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced, action patterns of initial hydrolysis products
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
best substrate, endo mechanism
action patterns of initial hydrolysis products, product formation, overview
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
product formation, overview: oligoarabinosides with degree of polymerization between 2 and 5 are the major products, but only trace amounts of free arabinose
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced, action patterns of initial hydrolysis products
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
best substrate, endo mechanism
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
best substrate, endo mechanism
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
best substrate, endo mechanism
major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
best substrate, endo mechanism
action patterns of initial hydrolysis products, product formation, overview
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
best substrate: linear 1,5-alpha-L-haze arabinan
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
i.e. debranched arabinan
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
-
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
AB063312
i.e. debranched arabinan
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
AB063312
i.e. debranched arabinan
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
-
-
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
-
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
-
F-11 and IFO 3022: best substrate, endo mechanism
end-product
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
-
F-11 best substrate
end-product, F-11: releases arabinose, arabinobiose, arabinotriose and higher oligosaccharides during hydrolysis, end-product are arabinose and arabinobiose
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
-
IFO 3134, linear 1,5-alpha-L-arabinan
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
Bacillus subtilis F-11
-
-
-
-
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
Bacillus subtilis F-11
-
F-11 and IFO 3022: best substrate, endo mechanism
end-product
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
Bacillus subtilis F-11
-
F-11 best substrate
end-product, F-11: releases arabinose, arabinobiose, arabinotriose and higher oligosaccharides during hydrolysis, end-product are arabinose and arabinobiose
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
F-11 and IFO 3022: best substrate, endo mechanism
-
-
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
F-11 best substrate
-
-
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
IFO 3022, best substrate
IFO 3022
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
Bacillus subtilis F-11
-
F-11 and IFO 3022: best substrate, endo mechanism
-
-
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
Bacillus subtilis F-11
-
F-11 best substrate
-
-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside + L-arabinose
-
-
-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside + L-arabinose
-
-
-
?
1,5-L-alpha-arabinoheptaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinotetraose
-
-
-
-
?
1,5-L-alpha-arabinoheptaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinotetraose
-
-
-
-
?
1,5-L-alpha-arabinopentaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinobiose
-
-
-
-
?
1,5-L-alpha-arabinopentaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinobiose
-
-
-
-
?
1,5-L-alpha-arabinotetraose + H2O
1,5-L-alpha-arabinobiose + 1,5-L-alpha-arabinobiose
-
-
-
-
?
1,5-L-alpha-arabinotetraose + H2O
1,5-L-alpha-arabinobiose + 1,5-L-alpha-arabinobiose
-
-
-
-
?
1,5-L-alpha-arabinotriose + H2O
L-arabinose + 1,5-L-alpha-arabinobiose
-
-
-
-
?
1,5-L-alpha-arabinotriose + H2O
L-arabinose + 1,5-L-alpha-arabinobiose
-
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
very poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
mild hydrolysis: no significant degradation, exhaustive enzymatic hydrolysis: extremely low degradation
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP3
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP4
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, intermediate enzymatic hydrolysis: L-arabinobiose and much less L-arabinose + L-arabinotriose, end-products after exhaustive enzymatic degradation
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP5
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinobiose + L-arabinotriose
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP8
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinotriose + L-arabinopentaose and L-arabinobiose + L-arabinohexaose and L-arabinotetraose
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
at very low rate, with high enzyme concentration
-
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
at very low rate, with high enzyme concentration
-
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
no or extremely low degradation
-
-
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
DP2
-
-
?
arabinogalactan + H2O
?
-
for optimal breakdown of potato arabinogalactan combined action of endo-arabinase and endo-galactanase is required
-
-
?
cell wall polysaccharide + H2O
L-arabinan
-
F-11, primary cell wall polysaccharides
-
-
?
cell wall polysaccharide + H2O
L-arabinan
Bacillus subtilis F-11
-
F-11, primary cell wall polysaccharides
-
-
?
debranched 1,5-alpha-L-arabinan + H2O
?
highest activity
-
-
?
debranched 1,5-alpha-L-arabinan + H2O
?
highest activity
-
-
?
debranched arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
highest activity
-
-
?
debranched arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
-
?
feruloylated oligosaccharide + H2O
ferulic acid + ?
-
from sugar beet pulp, SBP
efficient releasing free ferulic acid from sugar beet pulp requires strong synergy involving three enzymes: endo-arabinanase, alpha-L-arabinofuranosidase and cinnamoyl esterase, free and esterified ferulic acid
?
feruloylated oligosaccharide + H2O
ferulic acid + ?
-
endo-arabinanase alone: very poor substrate, endo-arabinanase and alpha-L-arabinofuranosidase together: good substrate, best: combination endo-arabinanase, alpha-L-arabinofuranosidase and cinnamoyl esterase
efficient releasing free ferulic acid from sugar beet pulp requires strong synergy involving three enzymes: endo-arabinanase, alpha-L-arabinofuranosidase and cinnamoyl esterase, free and esterified ferulic acid
?
galactan + H2O
?
-
potato galactan: poor substrate, citrus galactan: very poor substrate
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
apple juice ultrafiltration retentate arabinan, branched sugar-beet arabinan, linear apple arabinan
end-product: low molecular weight oligomeric products
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
linear and branched arabinan
end-product: low molecular weight oligomeric products
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
random hydrolysis of L-arabinan
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
apple and sugar beet pulp
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
sugar beet pulp
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
apple juice ultrafiltration retentate arabinan, branched sugar-beet arabinan, linear apple arabinan
end-product: low molecular weight oligomeric products
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
beet and apple juice ultrafiltration retentate, UFR, arabinan
end-product: L-arabinose disaccharide + L-arabinose trisaccharide, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
sugar beet pulp
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
sugar beet pulp
end-product: L-arabinose disaccharide + L-arabinose trisaccharide, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
debranched and branched sugar beet arabinan
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
linear and branched arabinan
end-product: low molecular weight oligomeric products
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
random hydrolysis of L-arabinan
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
random hydrolysis of L-arabinan
end-product: L-arabinose disaccharide + L-arabinose trisaccharide, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced
?
L-arabinan + H2O
L-arabino-oligosaccharides
Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49
-
sugar beet pulp
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
sugar beet pulp
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
branched and partially debranched arabinan
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
sugar-beet arabinan in commercial apple juice concentrate
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
random hydrolysis of L-arabinan
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
random hydrolysis of L-arabinan
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
Bacillus subtilis F-11
-
sugar-beet arabinan in commercial apple juice concentrate
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
Bacillus subtilis F-11
-
branched and partially debranched arabinan
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
Clostridium felsineum var. sikokianum
-
random hydrolysis of L-arabinan
-
-
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
-
main product: L-arabinotriose
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
linear arabinan
main product: L-arabinotriose
?
L-arabinan + H2O
L-arabino-oligosaccharides
-
branched sugar beet arabinan containing alpha-1,3-arabinose side chains: not a substrate
main product: L-arabinotriose
?
L-arabino-oligosaccharide + H2O
L-arabino-oligosaccharide
-
-
-
?
L-arabino-oligosaccharide + H2O
L-arabino-oligosaccharide
-
-
product formation, small oligomeric products
?
L-arabino-oligosaccharide + H2O
L-arabino-oligosaccharide
-
-
product formation, small oligomeric products
?
L-arabino-oligosaccharide + H2O
L-arabino-oligosaccharide
-
degree of polymerization 2-8, DP2-8
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, L-arabinose + L-arabinobiose: end-products after exhaustive enzymatic degradation, product formation
?
L-arabino-oligosaccharide + H2O
L-arabino-oligosaccharide
-
linear 1,5-alpha-L-arabino-oligosaccharide
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, L-arabinose + L-arabinobiose: end-products after exhaustive enzymatic degradation, product formation
?
L-arabino-oligosaccharide + H2O
L-arabino-oligosaccharide
-
-
main product: L-arabinotriose
?
linear 1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
linear 1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
linear arabinan + H2O
?
-
hydrolyzed at 62% compared to debranched arabinan
-
-
?
linear arabinan + H2O
?
the enzyme is selective for linear arabinan
-
-
?
linear arabinan + H2O
?
the enzyme is selective for linear arabinan
-
-
?
protopectin + H2O
pectin
-
IFO 3134, protopectin of sugar beet pulp and various citrus fruit peels, but much less on that from lemon peel
IFO 3134, highly polymerized pectin
?
protopectin + H2O
pectin
-
IFO 3134, protopectin of sugar-beet and apple pulp
IFO 3134, soluble pectin
?
red debranched arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
red debranched arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
sugar beet arabinan + H2O
?
the substrate is composed of 1,2-alpha- and 1,3-alpha-L-arabinofuranosyl side chains linked to a 1,5-alpha-L-arabinofuranosyl backbone
-
-
?
sugar beet arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
sugar beet arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
sugar beet arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
-
?
sugar beet arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside + L-arabinose
-
-
-
?
sugar beet arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside + L-arabinose
-
-
-
?
additional information
?
-
-
substrate binding site, action pattern, subsite mapping
-
-
?
additional information
?
-
-
no bi-substrate reactions such as condensation or transglycosylation
-
-
?
additional information
?
-
-
inactive on p-nitrophenylarabinofuranoside, onion arabinogalactan, stractan, wheat arabinoxylan, oat spelts arabinoxylan, avicel, polygalacturonic acid
-
-
?
additional information
?
-
-
inactive on p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
additional information
?
-
-
substrate binding site, action pattern, subsite mapping
-
-
?
additional information
?
-
-
no bi-substrate reactions such as condensation or transglycosylation
-
-
?
additional information
?
-
-
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times
-
-
?
additional information
?
-
-
inactive towards synthetic arabinosides, p-nitrophenyl-alpha-L-arabinoside, arabinose disaccharide, arabinogalactan, stractan and xylan from oat spelts
-
-
?
additional information
?
-
the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated
-
-
?
additional information
?
-
the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated
-
-
?
additional information
?
-
-
no activity with: laminarin, lichenan, galactomannan, beta-glucan, wheat and rye arabinoxylan, xyloglucan, carboxymethyl cellulose, pectin from fruits, larch arabinogalactan, galactan (potato), xylan from beechwood, and 1,4-beta-mannan
-
-
?
additional information
?
-
the enzyme shows a synergic effect with the commercial cocktail Accellerase 1500 on cellulose hydrolysis. It weakens the interaction between cellulose fibers in filter paper, thus providing an increased access to the cellulases of the cocktail
-
-
?
additional information
?
-
-
the enzyme shows a synergic effect with the commercial cocktail Accellerase 1500 on cellulose hydrolysis. It weakens the interaction between cellulose fibers in filter paper, thus providing an increased access to the cellulases of the cocktail
-
-
?
additional information
?
-
the enzyme shows a synergic effect with the commercial cocktail Accellerase 1500 on cellulose hydrolysis. It weakens the interaction between cellulose fibers in filter paper, thus providing an increased access to the cellulases of the cocktail
-
-
?
additional information
?
-
the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated
-
-
?
additional information
?
-
the enzyme randomly cleaves the internal alpha-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated
-
-
?
additional information
?
-
-
IFO 3022: potato disc macerating activity, able to loosen potato tissue
-
-
?
additional information
?
-
-
IFO 3022: potato disc macerating activity, able to loosen potato tissue
-
-
?
additional information
?
-
-
IFO 3022: inactive on p-nitrophenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-D-galactopyranoside, p-nitrophenyl beta-D-galactopyranoside, arabinogalactan, carboxymethyl cellulose, filter paper, pectin and polygalacturonic acid
-
-
?
additional information
?
-
-
F-11: inactive on phenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-D-galactopyranoside, p-nitrophenyl beta-D-galactopyranoside, arabinoxylan, arabinogalactan and gum arabic
-
-
?
additional information
?
-
-
IFO 3134, inactive on polygalacturonic acid and soluble pectin
-
-
?
additional information
?
-
-
inactive towards phenyl and p-nitrophenyl alpha-L-arabinofuranoside, arabinoxylan, arabinogalactan, and gum arabic
-
-
?
additional information
?
-
-
plays a role in degradation of plant tissues, macerating enzyme
-
-
?
additional information
?
-
shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
additional information
?
-
-
shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
additional information
?
-
shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
additional information
?
-
Bacillus subtilis F-11
-
IFO 3022: potato disc macerating activity, able to loosen potato tissue
-
-
?
additional information
?
-
Bacillus subtilis F-11
-
inactive towards phenyl and p-nitrophenyl alpha-L-arabinofuranoside, arabinoxylan, arabinogalactan, and gum arabic
-
-
?
additional information
?
-
Bacillus subtilis F-11
-
F-11: inactive on phenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-D-galactopyranoside, p-nitrophenyl beta-D-galactopyranoside, arabinoxylan, arabinogalactan and gum arabic
-
-
?
additional information
?
-
-
the enzyme fails to cleave 1,5-alpha-arabinobiose, branched arabinan, arabinoxylan, arabinogalactan, xylan, and galactan
-
-
?
additional information
?
-
-
the enzyme fails to cleave 1,5-alpha-arabinobiose, branched arabinan, arabinoxylan, arabinogalactan, xylan, and galactan
-
-
?
additional information
?
-
no activity for arabinoxylan, 1,4-beta-D-xylose units with side chains of 1,3-alpha-L-arabinose, xylan, starch, or 4-nitrophenyl-alpha-L-arabinofuranoside, a typical substrate for the arabinofuranosidase. Synergistic hydroysis of arabinan by endo-1,5-alpha-L-arabinanase and alpha-L-arabinofuranosidase
-
-
?
additional information
?
-
AB063312
no substrates: carboxymethyl cellulose, xylan, polygalacturonate, pectin, galactan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside,4-nitrophenyl-alpha-D-xylopyranoside and 4-nitrophenyl-beta-D-cellobioside
-
-
?
additional information
?
-
-
no substrates: carboxymethyl cellulose, xylan, polygalacturonate, pectin, galactan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside,4-nitrophenyl-alpha-D-xylopyranoside and 4-nitrophenyl-beta-D-cellobioside
-
-
?
additional information
?
-
AB063312
no substrates: carboxymethyl cellulose, xylan, polygalacturonate, pectin, galactan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside,4-nitrophenyl-alpha-D-xylopyranoside and 4-nitrophenyl-beta-D-cellobioside
-
-
?
additional information
?
-
-
alkali extract of cell walls of Gossypium hirsutum is sequentially digested by endo-polygalacturonase, arabinofuranosidase, endo-arabinase, and rhamnogalacturonan hydrolase AN9314.2
-
-
?
additional information
?
-
no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch
-
-
?
additional information
?
-
no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch
-
-
?
additional information
?
-
does not hydrolyze birchwood xylan and 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
additional information
?
-
-
does not hydrolyze birchwood xylan and 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
additional information
?
-
-
inactive on cellulose, avicel, amorphous cellulose, carboxymethylcellulose, xylan, galactan, mannan, 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-xyloside and 2,4-dinitrophenyl beta-xylobioside
-
-
?
additional information
?
-
-
there is no activity towards 1,4-beta-D-mannan, galactan, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
additional information
?
-
no substrates: birchwood xylan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, and 4-nitrophenyl-beta-D-xylopyranoside
-
-
?
additional information
?
-
no activity with birchwood xylan, beechwood xylan, citric pectin and arabinogalactan
-
-
?
additional information
?
-
-
no activity with birchwood xylan, beechwood xylan, citric pectin and arabinogalactan
-
-
?
additional information
?
-
-
the enzyme shows the ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides
-
-
?
additional information
?
-
the enzyme acts on branched arabinan (from sugar beet), but more slowly when compared to linear or debranched arabinan
-
-
?
additional information
?
-
-
the enzyme acts on branched arabinan (from sugar beet), but more slowly when compared to linear or debranched arabinan
-
-
?
