Any feedback?
Literature summary for 3.2.1.99 extracted from
- Cloning and characterization of a cold-adapted endo-1,5-alpha-L-arabinanase from Paenibacillus polymyxa and rational design for acidic applicability (2014), J. Agric. Food Chem., 62, 8460-8469.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Paenibacillus polymyxa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H218D | the mutant shows higher catalytic efficiency, kcat is improved about 45% versus that of the wild type enzyme | Paenibacillus polymyxa |
| H218E | the mutant is active, but it does not change the enzymatic property obviously under acidic condition compared with the wild type enzyme | Paenibacillus polymyxa |
| H218K | inactive | Paenibacillus polymyxa |
| H218R | inactive | Paenibacillus polymyxa |
| H297D | inactive | Paenibacillus polymyxa |
| H297E | inactive | Paenibacillus polymyxa |
| H297K | inactive | Paenibacillus polymyxa |
| H297R | inactive | Paenibacillus polymyxa |
| H48D | inactive | Paenibacillus polymyxa |
| H48E | inactive | Paenibacillus polymyxa |
| H48K | inactive | Paenibacillus polymyxa |
| H48R | inactive | Paenibacillus polymyxa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1177 | - |
linear 1,5-alpha-L-arabinan | at 40°C and pH 6.0 | Paenibacillus polymyxa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35477 | - |
x * 35477, calculated from amino acid sequence | Paenibacillus polymyxa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus polymyxa | V5LER1 | - |
- |
| Paenibacillus polymyxa Z6 | V5LER1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Paenibacillus polymyxa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 73.4 | - |
with linear linear 1,5-alpha-L-arabinan as substrate, at 40°C, pH 6.0 | Paenibacillus polymyxa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linear 1,5-alpha-L-arabinan + H2O | - |
Paenibacillus polymyxa | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
| linear 1,5-alpha-L-arabinan + H2O | - |
Paenibacillus polymyxa Z6 | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
| additional information | no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch | Paenibacillus polymyxa | ? | - |
? | |
| additional information | no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch | Paenibacillus polymyxa Z6 | ? | - |
? | |
| red debranched arabinan + H2O | - |
Paenibacillus polymyxa | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
| red debranched arabinan + H2O | - |
Paenibacillus polymyxa Z6 | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
| sugar beet arabinan + H2O | - |
Paenibacillus polymyxa | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
| sugar beet arabinan + H2O | - |
Paenibacillus polymyxa Z6 | oligo-alpha-(1->5)-L-arabinoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35477, calculated from amino acid sequence | Paenibacillus polymyxa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AbnZ1 | - |
Paenibacillus polymyxa |
| endo-1,5-alpha-L-arabinase | - |
Paenibacillus polymyxa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Paenibacillus polymyxa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
the specific activity of the enzyme remains 54.1% of maximum at 5°C | Paenibacillus polymyxa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 68.7 | - |
linear 1,5-alpha-L-arabinan | wild type enzyme, at 40°C and pH 6.0 | Paenibacillus polymyxa | |
| 99.7 | - |
linear 1,5-alpha-L-arabinan | mutant enzyme H218D, at 40°C and pH 6.0 | Paenibacillus polymyxa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Paenibacillus polymyxa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | the enzyme achieves about 20.4 and 83.6% of the maximal activity at pH 5.0 and 7.0, respectively | Paenibacillus polymyxa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | the enzyme is stable at pH 7.0 compared with 55.5 and 5.78% of maximal activity remaining after 30 min of incubation at pH 6.0 and 5.0 respectively | Paenibacillus polymyxa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Paenibacillus polymyxa | calculated from amino acid sequence | - |
9.2 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
