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3.2.1.70: glucan 1,6-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,6-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.70

Reaction

isomaltoheptaose
+
H2O
=
isomaltohexaose
+
D-glucose

Synonyms

1,6-alpha-D-glucan glucohydrolase, alpha-1,6-glucosidase, dextran glucosidase, DGase, exo-1,6-alpha-glucosidase, exodextranase, GH13_31 glucan 1,6-alpha-glucosidase, glucodextranase, glucose-forming exodextranase, LaGH13_31, MalL, oligo-alpha-1,6-glucosidase, SMDG, TreX

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.70 glucan 1,6-alpha-glucosidase

Cloned

Cloned on EC 3.2.1.70 - glucan 1,6-alpha-glucosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
dexB, sequence comparisons
dexB, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) CodonPlus RIL
expressed in Escherichia coli BL21 (DE3) CodonPlus-RIL cells
expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells
-
expression in Escherichia coli
gene LBA0264, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic tree, the enzyme is encoded in the maltooligosaccharide operon, semiquantitative PCR expression analysis, cloning in Escherichia coli strain TOP10, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3) cells
-
wild-type and mutant enzymes produced in Escherichia coli
wild-type and mutant expressed in Escherichia coli BL21 (DE3) CodonPlus RIL containing the expression plasmid derived from pET23d