Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the addition of Ca2+ (optimum 5 mM) enhances the thermostability of dextran glucosidase but has no significant effect on catalytic reaction | Streptococcus mutans | |
Mg2+ | the addition of Mg2+ (optimum 15 mM) enhances the thermostability of dextran glucosidase | Streptococcus mutans | |
additional information | K+ and Na+ do not enhance the thermostability of dextran glucosidase | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | Streptococcus mutans | - |
beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Streptococcus mutans | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
dextran + H2O | - |
Streptococcus mutans | beta-D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dextran glucosidase | - |
Streptococcus mutans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
the enzyme activity drops to 50% in 5 min at 46°C at 0-1 mM Ca2+, but the presence of 2 mM Ca2+ prolongs this to 30 min and 3-30 mM Ca2+ stabilizes the enzyme so that it retains 70% activity or higher even after 60 min incubation at 46°C | Streptococcus mutans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | the enzyme is stable between pH 5.5 and 7.0 in the presence of 5 mM Ca2+ after 15 min at 37°C | Streptococcus mutans |
5.8 | 6.7 | the enzyme is stable between pH 5.8 and 6.7 in the absence of Ca2+ after 15 min at 37°C | Streptococcus mutans |