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3.2.1.70: glucan 1,6-alpha-glucosidase

This is an abbreviated version!
For detailed information about glucan 1,6-alpha-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.70

Reaction

isomaltoheptaose
+
H2O
=
isomaltohexaose
+
D-glucose

Synonyms

1,6-alpha-D-glucan glucohydrolase, alpha-1,6-glucosidase, dextran glucosidase, DGase, exo-1,6-alpha-glucosidase, exodextranase, GH13_31 glucan 1,6-alpha-glucosidase, glucodextranase, glucose-forming exodextranase, LaGH13_31, MalL, oligo-alpha-1,6-glucosidase, SMDG, TreX

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.70 glucan 1,6-alpha-glucosidase

Temperature Stability

Temperature Stability on EC 3.2.1.70 - glucan 1,6-alpha-glucosidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
purified recombinant His6-tagged enzyme, pH 6.0, inactivation rate constant is 0.0001/min, T1/2 at 37°C is 4.8 days
40
15 min, pH 6.0, stable below
46
-
the enzyme activity drops to 50% in 5 min at 46°C at 0-1 mM Ca2+, but the presence of 2 mM Ca2+ prolongs this to 30 min and 3-30 mM Ca2+ stabilizes the enzyme so that it retains 70% activity or higher even after 60 min incubation at 46°C
60
-
pH 7.2, stable up to, wild type and several deletion mutants