3.2.1.57: isopullulanase
This is an abbreviated version!
For detailed information about isopullulanase, go to the full flat file.
Word Map on EC 3.2.1.57
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3.2.1.57
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pullulans
-
aspergillus
-
niger
-
ipu
-
glucoamylase
-
oligosaccharides
-
subsite
-
minioluteum
-
dextranases
-
penicillium
-
deglycosylation
-
neopullulanase
-
glucosidic
-
hydrolyse
-
dextran
-
flask
-
oryzae
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polysaccharide
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melanogenum
-
endoglycosidase
-
aureobasidium
-
peptide-n-glycosidase
- 3.2.1.57
- pullulans
- aspergillus
- niger
- ipu
- glucoamylase
- oligosaccharides
-
subsite
- minioluteum
- dextranases
-
penicillium
-
deglycosylation
- neopullulanase
-
glucosidic
-
hydrolyse
- dextran
-
flask
- oryzae
- polysaccharide
- melanogenum
-
endoglycosidase
-
aureobasidium
-
peptide-n-glycosidase
Reaction
hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose) =
Synonyms
IPU, More, pullulan 4-glucanohydrolase
ECTree
3.2.1.57 isopullulanaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.57 - isopullulanase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4(2)-alpha-isomaltosylisomaltose + H2O
?
-
cleaves the alpha-1,4-glycosidic linkage of the panose motif
-
-
?
4(3)-alpha-panosylpanose + H2O
?
-
cleaves the alpha-1,4-glycosidic linkage of the panose motif
-
-
?
6(2)-alpha-isomaltosylmaltose + H2O
?
-
isomaltotriosylglucose, cleaves the alpha-1,4-glycosidic linkage of the panose motif
-
-
?
6(2)-alpha-maltosylmaltose + H2O
?
-
cleaves the alpha-1,4-glycosidic linkage of the panose motif
-
-
?
6(3)-alpha-glucosylmaltotriose + H2O
?
-
cleaves the alpha-1,4-glycosidic linkage of the panose motif
-
-
?
panose + H2O
isomaltose + glucose
-
cleaves the alpha-1,4-glycosidic linkage
-
-
?
pullulan + H2O
isopanose
the enzyme cleaves the alpha(1,4) glucosidic linkage using an inverting mechanism and liberating D-glucose
trisaccharide [Glc-alpha(1,4)-Glc-alpha(1,6)-Glc]
-
?
pullulan + H2O
isopanose
the enzyme cleaves the alpha(1,4) glucosidic linkage using an inverting mechanism and liberating D-glucose
trisaccharide [Glc-alpha(1,4)-Glc-alpha(1,6)-Glc]
-
?
pullulan + H2O
isopanose
-
very strict substrate specificity, preferred over panose
-
-
?
pullulan + H2O
isopanose
-
IPU degrades alpha-1,4-glycosidic linkage from the reducing end adjacent to alpha-1,6-glycosidic linkage in pullulan
-
-
?
pullulan + H2O
isopanose
-
IPU degrades alpha-1,4-glycosidic linkage from the reducing end adjacent to alpha-1,6-glycosidic linkage in pullulan
-
-
?
pullulan + H2O
isopanose + ?
Asp353, Asp372 and Asp373 are the catalytic residues of IPU
-
-
?
pullulan + H2O
isopanose + ?
Asp353, Asp372 and Asp373 are the catalytic residues of IPU
-
-
?
additional information
?
-
-
recombinant IPU hydrolyzes various substrates containing the structure of panose indicating a strict subsite recognition of the panose motif, cleaves the alpha-1,4-glycosidic linkage
-
-
?
additional information
?
-
-
not: amylose, maltoheptaose, starch, amylopectin
-
-
?
additional information
?
-
-
not: amylose, maltoheptaose, starch, amylopectin
-
-
?
