3.2.1.57: isopullulanase

This is an abbreviated version!
For detailed information about isopullulanase, go to the full flat file.

hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose) =

IPU, More, pullulan 4-glucanohydrolase

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Results	in table
3	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
2	Crystallization (Commentary)
2	General Information
7	Inhibitors
5	KM Value [mM]
4	Localization
9	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
18	Organism
1	Oxidation Stability
10	PDB ID
10	pH Optimum
3	pH Range
7	pH Stability
1	pI Value
8	Posttranslational Modification
15	Protein Variants
9	Purification (Commentary)
1	Reaction
1	Reaction Type
18	Reference
5	Specific Activity [micromol/min/mg]
1	Storage Stability
36	Substrates and Products (Substrate)
5	Subunits
14	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
21	Temperature Stability [°C]
2	Turnover Number [1/s]

Cloned on EC 3.2.1.57 - isopullulanase

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expressed in Escherichia coli DH5alpha cells

expression of wild-type and mutant enzymes in Pichia pastoris strain GS115

ipuA gene, expression in Aspergillus oryzae M-2-3

ipuA gene, expression in Pichia pastoris GS115

recombinant IPU produced by Pichia pastoris