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3.2.1.57: isopullulanase

This is an abbreviated version!
For detailed information about isopullulanase, go to the full flat file.

Word Map on EC 3.2.1.57

Reaction

hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose) =

Synonyms

IPU, More, pullulan 4-glucanohydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.57 isopullulanase

Temperature Stability

Temperature Stability on EC 3.2.1.57 - isopullulanase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
F2, below, 30 min at pH 3.5
40
-
deglycosylated recombinant IPU, stable up to 40°C, native IPU has a similar thermal stability
45
-
F1, below, 30 min at pH 3.5
46.6
50% activity remaining for deglycosylated mutant N448A and for mutant Y440A
49.3
50% activity remaining for mutant N448A
52
50% activity remaining for deglycosylated mutant S450A
53
50% activity remaining for the deglycosylated wild-type enzyme
54
50% activity remaining for mutant S450A
90
-
pH 5, half-life: 20 min
additional information
-
calcium does not increase the thermostability