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Literature summary for 3.2.1.57 extracted from

  • Aoki, H.; Yopi; Sakano, Y.
    Molecular cloning and heterologous expression of the isopullulanase gene from Aspergillus niger A.T.C.C. 9642 (1997), Biochem. J., 323, 757-764.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.57

Localization

Localization Comment Organism GeneOntology No. Textmining
cell bound
-
 Aspergillus niger
-
-
extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
-
 Aspergillus niger
91000
-
 recombinant enzyme, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pullulan + H2O Aspergillus niger
-
 isopanose + (Glc)4
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 ATCC 9642, IPU F1 and IPU F2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylated Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
panose + H2O
-
 Aspergillus niger isomaltose + glucose
-
 ?
pullulan + H2O
-
 Aspergillus niger isopanose + (Glc)4
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 recombinant enzyme Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 recombinant enzyme Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 recombinant enzyme, at 4°C 12 h Aspergillus niger