3.2.1.40: alpha-L-rhamnosidase

This is an abbreviated version!
For detailed information about alpha-L-rhamnosidase, go to the full flat file.

Reaction

Synonyms

alpha-L-rhamnosidase, alpha-L-rhamnosidase A, alpha-L-rhamnosidase B, alpha-L-rhamnosidase N, alpha-L-rhamnosidase Ram A, alpha-L-rhamnosidase T, alpha-RHA, AoRha, AorhaA, BtRha, BtRha78A, DtRha, EC 3.2.1.66, GH106 alpha-L-rhamnosidase, gypenoside-alpha-L-rhamnosidase, KoRha, L-rhamnosidase, More, naringinase, pnp-rhamnohydrolase, RamA, RHA-P, RhaA, RhaB, RhaB1, RhaB2, RhaL1, Rham, rhamnosidase, alpha -L-, RhmA, saponin-alpha-L-rhamnosidase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.40 alpha-L-rhamnosidase

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Results	in table
3	Activating Compound
14294	AA Sequence
37	Application
1	CAS Registry Number
23	Cloned(Commentary)
4	Crystallization (Commentary)
6	Expression
4	General Stability
6	IC50 Value
146	Inhibitors
29	kcat/KM [mM/s]
22	Ki Value [mM]
81	KM Value [mM]
13	Localization
31	Metals/Ions
39	Molecular Weight [Da]
30	Natural Substrates/ Products (Substrates)
9	Organic Solvent Stability
135	Organism
1	Pathway
7	PDB ID
55	pH Optimum
24	pH Range
21	pH Stability
10	pI Value
12	Posttranslational Modification
50	Protein Variants
39	Purification (Commentary)
1	Reaction
1	Reaction Type
103	Reference
22	Source Tissue
30	Specific Activity [micromol/min/mg]
4	Storage Stability
309	Substrates and Products (Substrate)
42	Subunits
51	Synonyms
1	Systematic Name
47	Temperature Optimum [°C]
23	Temperature Range [°C]
68	Temperature Stability [°C]
31	Turnover Number [1/s]

Organic Solvent Stability

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Organic Solvent Stability on EC 3.2.1.40 - alpha-L-rhamnosidase

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Acetone

Novosphingobium sp. PP1Y

F6IEX3

10% acetone, 50 mM MOPS, pH 6.9, 6.73% residual activity. Complete loss of activity in 50% acetone

751348

CCl4

Penicillium sp.

-

immobilized naringinase is stable

136389

CH2Cl2

Penicillium sp.

-

immobilized naringinase is stable

136389

CHCl3

Penicillium sp.

-

immobilized naringinase is stable

136389

cyclohexane

Penicillium sp.

-

immobilized naringinase is stable

136389

DMSO

Novosphingobium sp. PP1Y

F6IEX3

10% DMSO, in 50 mM MOPS, pH 6.9, 66.29% residual activity. Complete loss of activity in 50% DMSO

751348

Ethanol

Novosphingobium sp. PP1Y

F6IEX3

10% ethanol, 50 mM MOPS, pH 6.9, 66.52% residual activity. Complete loss of activity in 50% ethanol

751348

ethylacetate

Penicillium sp.

-

immobilized naringinase is stable

136389

toluene

Penicillium sp.

-

immobilized naringinase is stable

136389