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3.2.1.40: alpha-L-rhamnosidase

This is an abbreviated version!
For detailed information about alpha-L-rhamnosidase, go to the full flat file.

Word Map on EC 3.2.1.40

Reaction

4',5,7-trihydroxyflavanone 7-rhamnoglucoside
+
H2O
=
4',5,7-trihydroxyflavanone 7-glucoside
+
alpha-L-rhamnose

Synonyms

alpha-L-rhamnosidase, alpha-L-rhamnosidase A, alpha-L-rhamnosidase B, alpha-L-rhamnosidase N, alpha-L-rhamnosidase Ram A, alpha-L-rhamnosidase T, alpha-RHA, AoRha, AorhaA, BtRha, BtRha78A, DtRha, EC 3.2.1.66, GH106 alpha-L-rhamnosidase, gypenoside-alpha-L-rhamnosidase, KoRha, L-rhamnosidase, More, naringinase, pnp-rhamnohydrolase, RamA, RHA-P, RhaA, RhaB, RhaB1, RhaB2, RhaL1, Rham, rhamnosidase, alpha -L-, RhmA, saponin-alpha-L-rhamnosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.40 alpha-L-rhamnosidase

Specific Activity

Specific Activity on EC 3.2.1.40 - alpha-L-rhamnosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
108.9
purified recombinant enzyme
13.3
-
60°C, pH 5.5
17.9
substrate 4-nitrophenyl alpha-L-rhamnopyranoside, pH 4.0, 40°C
2.44
-
purified enzyme
2.89
-
purified enzyme
21.7
-
purified enzyme, pH 5.5, 50°C
224.4
-
pH 4.5, 65°C
263.9
purified recombinant enzyme
29.5
substrate hesperidin, pH 4.0, 40°C
454
substrate: 4-nitrophenyl alpha-L-rhamnopyranoside, pH 6.5, 65°C, wild-type enzyme
486
substrate: 4-nitrophenyl alpha-L-rhamnopyranoside, pH 6.5, 65°C, mutant enzyme D594Q/G827K
504
substrate: 4-nitrophenyl alpha-L-rhamnopyranoside, pH 6.5, 65°C, mutant enzyme D594Q
526
-
pH 9.5, 37°C
711.9
activity of the recombinant overexpressed enzyme, pH and temperature not specified in the publication
82
-
p-nitrophenyl-alpha-L-rhamnopyranoside
85.3
substrate naringin, pH 4.0, 40°C
89.9
Bacteroides JY-6
-
-
additional information