Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.40: alpha-L-rhamnosidase

This is an abbreviated version!
For detailed information about alpha-L-rhamnosidase, go to the full flat file.

Word Map on EC 3.2.1.40

Reaction

4',5,7-trihydroxyflavanone 7-rhamnoglucoside
+
H2O
=
4',5,7-trihydroxyflavanone 7-glucoside
+
alpha-L-rhamnose

Synonyms

alpha-L-rhamnosidase, alpha-L-rhamnosidase A, alpha-L-rhamnosidase B, alpha-L-rhamnosidase N, alpha-L-rhamnosidase Ram A, alpha-L-rhamnosidase T, alpha-RHA, AoRha, AorhaA, BtRha, BtRha78A, DtRha, EC 3.2.1.66, GH106 alpha-L-rhamnosidase, gypenoside-alpha-L-rhamnosidase, KoRha, L-rhamnosidase, More, naringinase, pnp-rhamnohydrolase, RamA, RHA-P, RhaA, RhaB, RhaB1, RhaB2, RhaL1, Rham, rhamnosidase, alpha -L-, RhmA, saponin-alpha-L-rhamnosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.40 alpha-L-rhamnosidase

Cloned

Cloned on EC 3.2.1.40 - alpha-L-rhamnosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli B834-DE3 containing pET3a-RhaB plasmid
expressed in Escherichia coli BL21 S1 cells
expressed in Escherichia coli BL21-DE3 pRIL cells, recombinant strain of Echerichia coli carrying the rhmA gene from Thermomicrobia bacterium, immobilization on Ca2+ alginate beads and packaging the beads onto a column to design a bioreactor for production of rhamnose from naringin
expressed in Escherichia coli strain HMS174-DE3, transformation with mutant plasmids, pD567N, pE572Q, pD579N, or pE841Q for mutant generation
expressed in Pichia pastoris AorhaA
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli, gene has no homology to other glycoside hydrolases
-
expression in Pichia pastoris
expression in Pichia pastoris GS115
expression of alpha-L-rhamnosidase in Pichia pastoris and Kluyveromyces lactis achieves high protein yields of up to 17.6 U/ml and 30.6 U/ml
expression of wild-type enzyme and mutant enzymes in Pichia pastoris
-
gene rhaM, contsruction of a genomic library, DNA and mino acid sequence determination and analysis, expression in Escherichia coli
gene rhmA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
gene rhmB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
overexpression in Escherichia coli BL21(DE3)
overexpression in Pichia pastoris GS115s
the recombinant protein is fused to a C-terminal His-tag and expressed in Escherichia coli BL21(DE3)