3.2.1.40: alpha-L-rhamnosidase
This is an abbreviated version!
For detailed information about alpha-L-rhamnosidase, go to the full flat file.
Word Map on EC 3.2.1.40
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3.2.1.40
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l-rhamnose
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decumbens
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debittering
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hesperidin
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prunin
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grapefruit
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albidus
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food industry
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erubescens
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eupenicillium
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rhamnosylated
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hesperidinase
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beta-d-glucosidase
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nutrition
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alpha-1,2
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gellan
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synthesis
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biotechnology
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industry
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drug development
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degradation
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pharmacology
- 3.2.1.40
- l-rhamnose
- decumbens
-
debittering
- hesperidin
- prunin
- grapefruit
- albidus
- food industry
- erubescens
- eupenicillium
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rhamnosylated
-
hesperidinase
- beta-d-glucosidase
- nutrition
-
alpha-1,2
- gellan
- synthesis
- biotechnology
- industry
- drug development
- degradation
- pharmacology
Reaction
Synonyms
alpha-L-rhamnosidase, alpha-L-rhamnosidase A, alpha-L-rhamnosidase B, alpha-L-rhamnosidase N, alpha-L-rhamnosidase Ram A, alpha-L-rhamnosidase T, alpha-RHA, AoRha, AorhaA, BtRha, BtRha78A, DtRha, EC 3.2.1.66, GH106 alpha-L-rhamnosidase, gypenoside-alpha-L-rhamnosidase, KoRha, L-rhamnosidase, More, naringinase, pnp-rhamnohydrolase, RamA, RHA-P, RhaA, RhaB, RhaB1, RhaB2, RhaL1, Rham, rhamnosidase, alpha -L-, RhmA, saponin-alpha-L-rhamnosidase
ECTree
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Inhibitors
Inhibitors on EC 3.2.1.40 - alpha-L-rhamnosidase
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(3S,4S)-3,4-dihydroxy-5-spirocyclopropyl-DELTA-pyrroline
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72% inhibition at 1 mM
1,10-phenanthroline
less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB1 decreases by 50%; less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB2 decreases by 50%
acetonitrile
1% (v/v), about 55% loss of activity, 5% (v/v), about 90% loss of activity
dimethyl sulfoxide
the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is ecommended to improve the hydrolysis reaction of naringin dihydrochalcone
DMSO
1% (v/v), about 45% loss of activity, 5% (v/v), about 75% loss of activity
ethylene glycol
1% (v/v), about 15% loss of activity, 5% (v/v), about 30% loss of activity
five-membered ring azasugars
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with L-rhamnose configuration, substitution at the nitrogen shifts the inhibition mechanism from mixed to competitive
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Isopropanol
1% (v/v), about 25% loss of activity, 5% (v/v), about 55% loss of activity
N,N-Dimethylformamide
1% (v/v), about 70% loss of activity, 5% (v/v), about 90% loss of activity
p-chloromercuribenzoic acid
0.1 mM, 50% residual activity
phosphoramidon
isoform RhaB1 shows 23.8% residual activity at 1 mM; isoform RhaB2 shows 42.5% residual activity at 1 mM
polyhexamethylene biguanide
1 mM reduces the activity of isoform RhaB1 to 10.9% and isoform RhaB2 to 36.0% residual activity; 1 mM reduces the activity of isoform RhaB1 to 10.9% residual activity
thiorphan
less effective inhibitor; less effective inhibitor
[4-([[(2S,3S,4R)-3,4-dihydroxypyrrolidin-2-yl]methyl]amino)-5-hydroxy-6-methylpyridin-2-yl]methyl dihydrogen phosphate
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[4-([[(2S,3S,4S)-3,4-dihydroxypyrrolidin-2-yl]methyl]amino)-5-hydroxy-6-methylpyridin-2-yl]methyl dihydrogen phosphate
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the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is ecommended to improve the hydrolysis reaction of naringin dihydrochalcone
acetone
1% (v/v), about 50% loss of activity, 5% (v/v), about 75% loss of activity
Cu2+
5 mM, 92% loss of activity. 1 mM, 83% loss of activity
1 mM, 4% residual activity; 1 mM, 70% residual activity
D-glucose
about 500 mM D-glucose concentration in grape juice inhibits RhmA by 39%; about 500 mM D-glucose concentration in grape juice inhibits RhmB by 38%
ethanol
1% (v/v), about 25% loss of activity, 5% (v/v), about 55% loss of activity
ethanol
at 12% v/v, 51% inhibition of RhmB; at 12% v/v, 60% inhibition of RhmA
L-rhamnose
10 mM, 14% residual activity; 10 mM, 37% residual activity
L-rhamnose
crystallized complex with interacting site determined, for kinetic assay different concentrations ranging between 0 and 100 mM of rhamnose used
L-rhamnose
isoform RhaB1 is slightly inhibited by L-rhamnose, showing noncompetitive inhibition
L-rhamnose
complete inhibition, competitive product inhibition; complete inhibition, competitive product inhibition
the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is recommended to improve the hydrolysis reaction of naringin dihydrochalcone
methanol
1% (v/v), about 15% loss of activity, 5% (v/v), about 50% loss of activity
SDS produces partial inactivation; SDS produces partial inactivation
Zn2+
5 mM, 32% loss of activity. 1 mM, 55% loss of activity
Zn2+
30-35% inhibition at 2 mM, reversible by 10 mM EDTA
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neither divalent cations such as Ca2+, Mg2+, Mn2+, and Co2+ nor reducing agents such as 2-mercaptoethanol and dithiothreitol show effects on enzyme activtiy
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additional information
dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity
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additional information
dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity
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additional information
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dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity
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additional information
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not inhibitory at 1 mM: EDTA, CaCl2, 2-mercaptoethanol
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additional information
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design from aromatic amino acids and inhibitory potency of polyhydroxylated pyrrolidines, stereochemistry, overview
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additional information
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not inhibited by deoxyrhamnojirimycin and 5-C-spirocyclopropyl-(5-demethyl-1-deoxy)-L-fuconojirimycin
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