Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.183: UDP-N-acetylglucosamine 2-epimerase (hydrolysing)

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine 2-epimerase (hydrolysing), go to the full flat file.

Word Map on EC 3.2.1.183

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
H2O
=
N-acetyl-D-mannosamine
 +
UDP

Synonyms

bifunctional enzyme UDP-Nacetylglucosamine 2-epimerase/N-acetylmannosamine kinase, bifunctional UDP-GlcNAc 2-epimerase/ManNAc kinase, bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, GNE, hydrolyzing epimerase, hydrolyzing UDP-GlcNAc 2-epimerase, More, neuC, SeMet UDP-GlcNAc 2-epimerase, siaA, UDP-GlcNAc 2-epimerase, UDP-GlcNAc 2-epimerase/ManNAc kinase, UDP-GlcNAc-2-epimerase, UDP-GlcNAc-2-epimerase/ManAc kinase, UDP-N-acetylglucosamine 2-epimerase, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-N-acetylglucosamine 2-epimerase/Nacetylmannosamine kinase, UDP-N-acetylglucosamine2-epimerase/N-acetylmannosamine kinase, uridine diphosphate-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, uridine diphosphate-N-acetylglucosamine-2-epimerase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.183 UDP-N-acetylglucosamine 2-epimerase (hydrolysing)

Cloned

Cloned on EC 3.2.1.183 - UDP-N-acetylglucosamine 2-epimerase (hydrolysing)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein
-
expressed as a His-tagged fusion protein in Escherichia coli
-
expression of recombinant wild-type and mutant enzymes
-
functionally expressed in Escherichia coli, the yeast strains Saccharomyces cerevisiae and Pichia pastoris, and insect cells. In Escherichia coli, up to 2mg protein/l cell culture is expressed, in yeast cells 0.4mg/l, up to 100 mg/l, are expressed in insect cells. In all three cell systems, insoluble protein aggregates are also observed
-
gene GNE
-
gene gne, genotyping, transcriptional analysis of the GNE variant c.717T>G, recombinant expression of splicing mutant c.717T>G in HEK 293 cells
gene gne, located on chromosome 9p13.3, genotyping
gene gne, overexpresion of wild-type and mutant enzymes in HEK-293 cells, the endogenous GNE is knocked down in HEK-293 cells using GNE-specific shRNA
gene gne, recombinant expression of N-terminally FLAG3-tagged wild-type and mutant M743T in HEK-293 cells
gene Gne1, sequence comparison with the human isozyme Gne1
gene Gne2, sequence comparison with the human isozyme Gne2
recombinant bifunctional enzyme is expressed in COS7 cells
-
UDP-GlcNAc 2-epimerase/ManNAc kinase from rat is overexpressed in Spodoptera frugiperda cells (Sf9 cells) using a baculovirus expression system
-