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Literature summary for 3.2.1.183 extracted from

  • Murkin, A.S.; Chou, W.K.; Wakarchuk, W.W.; Tanner, M.E.
    Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase (2004), Biochemistry, 43, 14290-14298.
    View publication on PubMed
Search for more literature for 3.2.1.183

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Neisseria meningitidis

Protein Variants

Protein Variants Comment Organism
D100N mutant is found to be significantly catalytically compromised (kcat reduced by 1000) Neisseria meningitidis
D131N mutant is found to be significantly catalytically compromised (kcat reduced by 1000), 2-acetamidoglucal is released from the active site during catalysis, providing direct evidence that the enzyme is capable of catalyzing the anti elimination of UDP from UDP-GlcNAc Neisseria meningitidis
E122Q mutant is found to be significantly catalytically compromised (kcat reduced by 1000), 2-acetamidoglucal is released from the active site during catalysis, providing direct evidence that the enzyme is capable of catalyzing the anti elimination of UDP from UDP-GlcNAc Neisseria meningitidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.49
-
 UDP-N-acetyl-alpha-D-glucosamine wild-type, 37°C, pH not specified in the publication Neisseria meningitidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 SDS-PAGE Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-
Neisseria meningitidis MC58 group B
-
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + H2O = N-acetyl-D-mannosamine + UDP the mechanism is proposed to involve an anti elimination of UDP to form 2-acetamidoglucal as an intermediate, followed by the syn addition of water Neisseria meningitidis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Neisseria meningitidis ?
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Neisseria meningitidis MC58 group B ?
-
 ?

Synonyms

Synonyms Comment Organism
neuC
-
 Neisseria meningitidis
siaA
-
 Neisseria meningitidis
UDP-N-acetylglucosamine 2-epimerase
-
 Neisseria meningitidis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Neisseria meningitidis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
 UDP-N-acetyl-alpha-D-glucosamine wild-type, 37°C, pH not specified in the publication Neisseria meningitidis