Literature summary for 3.2.1.183 extracted from

Blume, A.; Ghaderi, D.; Liebich, V.; Hinderlich, S.; Donner, P.; Reutter, W.; Lucka, L.
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, functionally expressed in and purified from Escherichia coli, yeast, and insect cells (2004), Protein Expr. Purif., 35, 387-396.

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Cloned(Commentary)

Cloned (Comment)	Organism
functionally expressed in Escherichia coli, the yeast strains Saccharomyces cerevisiae and Pichia pastoris, and insect cells. In Escherichia coli, up to 2mg protein/l cell culture is expressed, in yeast cells 0.4mg/l, up to 100 mg/l, are expressed in insect cells. In all three cell systems, insoluble protein aggregates are also observed	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification from Escherichia coli results in 100 microgram/l pure and structurally intact protein. For insect cells, purification methods are established which result in up to 50 mg/l pure, soluble, and active protein	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + H2O	-	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
UDP-GlcNAc 2-epimerase	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Rattus norvegicus

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Release 2023.1 (January 2023)