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3.11.1.2: phosphonoacetate hydrolase

This is an abbreviated version!
For detailed information about phosphonoacetate hydrolase, go to the full flat file.

Word Map on EC 3.11.1.2

Reaction

Phosphonoacetate
+
H2O
=
acetate
+
phosphate

Synonyms

Hydrolase, phosphonoacetate, PAc hydrolase, PhnA

ECTree

     3 Hydrolases
         3.11 Acting on carbon-phosphorus bonds
             3.11.1 Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
                3.11.1.2 phosphonoacetate hydrolase

Purification

Purification on EC 3.11.1.2 - phosphonoacetate hydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli strain JM105 by ammonium sulfate fractionation, and anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, followed by gel filtration
-
recombinant N-terminally His6-tagged PhnA from Escherichia coli by nickel affinity chromatography
-