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Literature summary for 3.11.1.2 extracted from

  • Borisova, S.A.; Christman, H.D.; Metcalf, M.E.; Zulkepli, N.A.; Zhang, J.K.; van der Donk, W.A.; Metcalf, W.W.
    Genetic and biochemical characterization of a pathway for the degradation of 2-aminoethylphosphonate in Sinorhizobium meliloti 1021 (2011), J. Biol. Chem., 286, 22283-22290.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene phnA, expression of N-terminally His6-tagged PhnA in Escherichia coli Sinorhizobium meliloti

Protein Variants

Protein Variants Comment Organism
additional information construction of a phnA deletion mutant Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics analysis Sinorhizobium meliloti
0.0221
-
Phosphonoacetate pH 7.5, 30°C, recombinant enzyme Sinorhizobium meliloti

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ increases kcat and Km, decreasing kcat/KM, in the reaction Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48400
-
x * 48400, recombinant His6-tagged PhnA, SDS-PAGE Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Phosphonoacetate + H2O Sinorhizobium meliloti
-
acetate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
gene phnA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged PhnA from Escherichia coli by nickel affinity chromatography Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Phosphonoacetate + H2O
-
Sinorhizobium meliloti acetate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 48400, recombinant His6-tagged PhnA, SDS-PAGE Sinorhizobium meliloti

Synonyms

Synonyms Comment Organism
PhnA
-
Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sinorhizobium meliloti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
Phosphonoacetate pH 7.5, 30°C, recombinant enzyme Sinorhizobium meliloti
1.1
-
Phosphonoacetate pH 7.5, 30°C, recombinant enzyme, with 0.01 mM Zn2+ Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Sinorhizobium meliloti

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a step in the 2-aminoethylphosphonate degradation, conversion of 2-aminoethylphosphonate to phosphate and degradation of phosphonate compounds, pathway overview Sinorhizobium meliloti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4100
-
Phosphonoacetate pH 7.5, 30°C, recombinant enzyme Sinorhizobium meliloti