Cloned (Comment) | Organism |
---|---|
gene phnA, expression of N-terminally His6-tagged PhnA in Escherichia coli | Sinorhizobium meliloti |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a phnA deletion mutant | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics analysis | Sinorhizobium meliloti | |
0.0221 | - |
Phosphonoacetate | pH 7.5, 30°C, recombinant enzyme | Sinorhizobium meliloti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | increases kcat and Km, decreasing kcat/KM, in the reaction | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48400 | - |
x * 48400, recombinant His6-tagged PhnA, SDS-PAGE | Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Phosphonoacetate + H2O | Sinorhizobium meliloti | - |
acetate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
gene phnA | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged PhnA from Escherichia coli by nickel affinity chromatography | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Phosphonoacetate + H2O | - |
Sinorhizobium meliloti | acetate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48400, recombinant His6-tagged PhnA, SDS-PAGE | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
PhnA | - |
Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sinorhizobium meliloti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
Phosphonoacetate | pH 7.5, 30°C, recombinant enzyme | Sinorhizobium meliloti | |
1.1 | - |
Phosphonoacetate | pH 7.5, 30°C, recombinant enzyme, with 0.01 mM Zn2+ | Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sinorhizobium meliloti |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes a step in the 2-aminoethylphosphonate degradation, conversion of 2-aminoethylphosphonate to phosphate and degradation of phosphonate compounds, pathway overview | Sinorhizobium meliloti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4100 | - |
Phosphonoacetate | pH 7.5, 30°C, recombinant enzyme | Sinorhizobium meliloti |