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Literature summary for 3.11.1.2 extracted from
- The purification and properties of phosphonoacetate hydrolase, a novel carbon-phosphorus bond-cleavage enzyme from Pseudomonas fluorescens 23F (1995), Eur. J. Biochem., 234, 225-230.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | activity stain for detection of carbon-phosphorus cleavage by phosphonoacetate hydrolase in PAGE gels | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, complete inhibition | Pseudomonas fluorescens |  |
| 3-phosphonopropionate | - |
Pseudomonas fluorescens | |
| EDTA | 5 mM, complete inhibition | Pseudomonas fluorescens | |
| Phosphonoacetate | substrate inhibition at 25 mM and above | Pseudomonas fluorescens | |
| Phosphonoformate | - |
Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.25 | - |
Phosphonoacetate | - |
Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | enhances activity | Pseudomonas fluorescens | |
| Mn2+ | enhances activity | Pseudomonas fluorescens | |
| Zn2+ | enhances activity, best activation at 1 mM | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Pseudomonas fluorescens |
| 80000 | - |
gel filtration | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens 23F | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:phosphonoacetate-grown cell | phosphonoacetate as the sole carbon and phosphorus source | Pseudomonas fluorescens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 35% loss of activity after 31 days, 45% loss of activity after 34 days | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Phosphonoacetate + H2O | - |
Pseudomonas fluorescens | acetate + phosphate | - |
? | |
| Phosphonoacetate + H2O | - |
Pseudomonas fluorescens 23F | acetate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | 20°C: about 40% of maximal activity, 45°C: about 50% of maximal activity | Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, rapid loss of activity | Pseudomonas fluorescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
Phosphonoacetate | - |
Pseudomonas fluorescens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.6 | pH 7.0: about 65% of maximal activity, pH 9.6: about 75% of maximal activity | Pseudomonas fluorescens |
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