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3.11.1.2: phosphonoacetate hydrolase

This is an abbreviated version!
For detailed information about phosphonoacetate hydrolase, go to the full flat file.

Word Map on EC 3.11.1.2

Reaction

Phosphonoacetate
+
H2O
=
acetate
 +
phosphate

Synonyms

Hydrolase, phosphonoacetate, PAc hydrolase, PhnA

ECTree

     3 Hydrolases
         3.11 Acting on carbon-phosphorus bonds
             3.11.1 Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
                3.11.1.2 phosphonoacetate hydrolase

Crystallization

Crystallization on EC 3.11.1.2 - phosphonoacetate hydrolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme in in the presence of Zn2+, Mg2+, phosphonoformate and tartrate, from 30 mM K+BICINE, pH 8.5, and 1 mM ZnCl2, mixed with 10% poly(ethylene glycol) 8000, 100 mM Mg-acetate, and 5 mM phosphonoformate buffered at pH 7.0 with 100 mM MOPSO, X-ray diffraction structure determination and analysis at 2.8 A resolution, multiple isomorphous replacement with two heavy-atom derivatives
-
selenomethionine-labeled enzyme, on-chip technology using a 24-well hybrid array chip, X-ray diffraction structure determination and analysis at 2.11 A resolution, crystallization screening and optimization, and usage of a microfluidic approach for the crystallization and de novo X-ray structure determination of novel targets via on-chip anomalous X-ray diffraction analysis, method validation
-
X-ray diffraction structure determination and analysis at 1.35 A resolution, cocrystal structures with phosphonoacetate substrate, acetate, phosphonoformate inhibitor, and a covalently bound transition state mimic