3.1.26.8: ribonuclease M5
This is an abbreviated version!
For detailed information about ribonuclease M5, go to the full flat file.
Reaction
endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor =
Synonyms
5S ribosomal maturation nuclease, 5S ribosomal RNA maturase, 5S ribosomal RNA maturation endonuclease, alkaline ribonuclease, nuclease, ribo-, nuclease, ribosomal ribonucleate maturation endoribo-, ribonuclease M5, ribosomal RNA maturation endonuclease, RNase M5
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Engineering
Engineering on EC 3.1.26.8 - ribonuclease M5
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additional information
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bacteria lacking the gene rnmV do not produce mature 5S RNA, but it is nonessential for cell viability and not necessary for ribosomal function
additional information
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construction of mutant strains which shows significantly altered expression of some genes, e.g. ctsR, due to mutated RNase M5 which does not process 5S pre-rRNA, the effect on odhAB and sucCD operons is indirect, overview