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3.1.26.8: ribonuclease M5

This is an abbreviated version!
For detailed information about ribonuclease M5, go to the full flat file.

Reaction

endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor =

Synonyms

5S ribosomal maturation nuclease, 5S ribosomal RNA maturase, 5S ribosomal RNA maturation endonuclease, alkaline ribonuclease, nuclease, ribo-, nuclease, ribosomal ribonucleate maturation endoribo-, ribonuclease M5, ribosomal RNA maturation endonuclease, RNase M5

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.8 ribonuclease M5

Engineering

Engineering on EC 3.1.26.8 - ribonuclease M5

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D14A
-
less than 10% of wild-type activity
D56A
-
less than 10% of wild-type activity
D58A
-
less than 10% of wild-type activity
E10A
-
less than 10% of wild-type activity
G11A
-
less than 10% of wild-type activity
G31A
-
less than 10% of wild-type activity
G61A
-
less than 10% of wild-type activity
I52A
-
60% of wild-type activity
I64A
-
as active as wild-type enzyme
L107A
-
less than 10% of wild-type activity
L162A
-
about 25% of wild-type activity
R65A
-
about 10% of wild-type activity
Y152A
-
as active as wild-type enzyme
additional information