Information on EC 3.1.26.8 - ribonuclease M5

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.26.8
-
RECOMMENDED NAME
GeneOntology No.
ribonuclease M5
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
CAS REGISTRY NUMBER
COMMENTARY hide
62253-00-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain Q
-
-
Manually annotated by BRENDA team
strain Q
-
-
Manually annotated by BRENDA team
strain 168
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5S precursor rRNA + H2O
mature 5S rRNA
show the reaction diagram
5S rRNA precursor + H2O
?
show the reaction diagram
5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5S precursor rRNA + H2O
mature 5S rRNA
show the reaction diagram
5S rRNA precursor + H2O
?
show the reaction diagram
additional information
?
-
-
the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
divalent cation, metal or polyamine is required
K+
-
activates, inhibition above 0.1 M
Mg2+
-
divalent cation, metal or polyamine is required
Mn2+
-
divalent cation, metal or polyamine is required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
mature 5S RNA
-
from Bacillus licheniformis, E. coli or Saccharomyces carlsbergensis
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Zn2+
-
inhibits in presence of Mg2+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NH4+
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activates, inhibition above 0.1 M
putrescine
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divalent cation, metal or polyamine is required, 0.01 M putrescine is as effective as Mg2+
spermidine
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divalent cation, metal or polyamine is required, 0.3 mM is as effective as Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00015
RNA
-
-
0.00003
RNA in complex with BL16 subunit
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-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00667
artificial 5S rRNA precursor
Bacillus subtilis
-
-
-
0.0567
artificial 5S rRNA precursor substrate in complex with BL16 subunit
Bacillus subtilis
-
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000
-
15000 Da is the MW of the beta component, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native from strain SSB306 containing a modified L6 protein, recombinant from Escherichia coli
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purification of beta-component
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RNase M5 mutant enzymes
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene yabF, overexpression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D14A
-
less than 10% of wild-type activity
D56A
-
less than 10% of wild-type activity
D58A
-
less than 10% of wild-type activity
E10A
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less than 10% of wild-type activity
G11A
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less than 10% of wild-type activity
G31A
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less than 10% of wild-type activity
G61A
-
less than 10% of wild-type activity
I52A
-
60% of wild-type activity
I64A
-
as active as wild-type enzyme
L107A
-
less than 10% of wild-type activity
L162A
-
about 25% of wild-type activity
R65A
-
about 10% of wild-type activity
Y152A
-
as active as wild-type enzyme
additional information
Show AA Sequence (2682 entries)
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