3.1.26.8: ribonuclease M5
This is an abbreviated version!
For detailed information about ribonuclease M5, go to the full flat file.
Reaction
endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor =
Synonyms
5S ribosomal maturation nuclease, 5S ribosomal RNA maturase, 5S ribosomal RNA maturation endonuclease, alkaline ribonuclease, nuclease, ribo-, nuclease, ribosomal ribonucleate maturation endoribo-, ribonuclease M5, ribosomal RNA maturation endonuclease, RNase M5
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Substrates Products
Substrates Products on EC 3.1.26.8 - ribonuclease M5
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REACTION DIAGRAM
artificial 5S rRNA precursor substrate in complex with BL16 subunit + H2O
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5S precursor rRNA + H2O
mature 5S rRNA
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enzyme is responsible for maturation of 5S rRNA
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5S precursor rRNA + H2O
mature 5S rRNA
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in contrast to the Escherichia coli enzyme, the Bacillus subtilis enzyme cleaves the 5S rRNA in a double-stranded region that extends from the L5 arm in one step
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5S precursor rRNA + H2O
mature 5S rRNA
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5S rRNA maturation
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responsible for terminal maturation of 5S rRNA
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5S rRNA precursor + H2O
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responsible for terminal maturation of 5S rRNA
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mature 5S rRNA + oligonucleotides
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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symmetrically arranged sequences are not used in the RNase M5 interaction
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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electrostatic interactions contribute about 65% to the overall binding energy
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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recognition elements in potential RNase M5 substrates mainly reside in the mature moiety of the precursor. The enzyme recognizes the overall conformation of potential substrates
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme attacks a ribonucleoprotein substrate consisting of protein BL16 in complex with the 5S rRNA precursor
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme cleaves twice in double-helical region of a 179-nucleotide precursor of 5S rRNA to yield mature 5S rRNA of 116 nucleotides plus fragments of 21 and 42 nucleotides. The conformation, not sequence, is the important feature in the locale of the cleaved bonds
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the 5'-precursor-specific segment contains one or more components recognized by RNase M5 during its interaction with the p5A substrate
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
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the artificial substrate lacking the 5'-terminal precursor segment is cleaved very much more slowly than that lacking 3' segment
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additional information
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the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution
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