Cloned (Comment) | Organism |
---|---|
gene yabF, overexpression in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | bacteria lacking the gene rnmV do not produce mature 5S RNA, but it is nonessential for cell viability and not necessary for ribosomal function | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5S precursor rRNA + H2O | Bacillus subtilis | enzyme is responsible for maturation of 5S rRNA | mature 5S rRNA | - |
? | |
additional information | Bacillus subtilis | the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene yabF, proposition to rename the gene rnmV | - |
Purification (Comment) | Organism |
---|---|
native from strain SSB306 containing a modified L6 protein, recombinant from Escherichia coli | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor | reaction scheme | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5S precursor rRNA + H2O | enzyme is responsible for maturation of 5S rRNA | Bacillus subtilis | mature 5S rRNA | - |
? | |
5S precursor rRNA + H2O | in contrast to the Escherichia coli enzyme, the Bacillus subtilis enzyme cleaves the 5S rRNA in a double-stranded region that extends from the L5 arm in one step | Bacillus subtilis | mature 5S rRNA | - |
? | |
additional information | the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5S ribosomal maturation nuclease | - |
Bacillus subtilis |
5S ribosomal RNA maturase | - |
Bacillus subtilis |
5S ribosomal RNA maturation endonuclease | - |
Bacillus subtilis |
RNase M5 | - |
Bacillus subtilis |