3.1.13.3: oligonucleotidase

This is an abbreviated version!
For detailed information about oligonucleotidase, go to the full flat file.

Word Map on EC 3.1.13.3

3.1.13.3

exoribonuclease

exonuclease

trna

oligoribonucleotides

pnpase

cyclic-di-gmp

exonucleolytic

phosphoesterase

c-di-gmp

deddh

3'-phosphoadenosine

Reaction

exonucleolytic cleavage of oligonucleotides to yield nucleoside 5'-phosphates =

Synonyms

CpsORN, EC 3.1.4.19, More, nanoRNase, NrnC, nucleotidase, oligo-, oligoribonuclease, ORN, phosphodiesterase, REXO2, RNase T, small fragment nuclease, XC847, YtqI

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.13 Exoribonucleases producing 5′-phosphomonoesters

3.1.13.3 oligonucleotidase

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Results	in table
202	AA Sequence
3	Application
1	CAS Registry Number
14	Cloned(Commentary)
6	Crystallization (Commentary)
21	Disease/ Diagnostics
36	General Information
27	Inhibitors
3	Ki Value [mM]
14	KM Value [mM]
3	Localization
25	Metals/Ions
7	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
41	Organism
13	pH Optimum
3	pH Range
26	Protein Variants
15	Purification (Commentary)
3	Reaction
1	Reaction Type
41	Reference
1	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
119	Substrates and Products (Substrate)
15	Subunits
53	Synonyms
9	Temperature Optimum [°C]
4	Temperature Stability [°C]
10	Turnover Number [1/s]

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Cloned on EC 3.1.13.3 - oligonucleotidase

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expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI

3 entries

expression in Escherichia coli

2 entries

expression of his-tagged YtqI under control of the arabinose-inducible promoter Para

Bacillus subtilis

682178

gene orn, detailed phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Pseudomonas aeruginosa

P57665

752033

gene orn, quantitative RT-PCR expression analysis

2 entries

gene orn, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha

Colwellia psychrerythraea

Q47VZ4

752249

into pMCSG7 vector

Xanthomonas campestris

682781

overexpression of the orn gene in Escherichia coli

Escherichia coli

134087, 134088

wild-type ornA and the ornA (D130A) point mutant are placed under the control of the strong, constitutive ermE promoter in the hygromycin-resistant, attP-integrative vector pIJ10275. The resultant plasmids (pJS93 and pJS94) introduced into Streptomyces coelicolor J3411 (DELTAornA::apr) by conjugation with Escherichia coli strain ET12567 (pUZ8002) to yield Streptomyces coelicolor J3411 (pJS93) and Streptomyces coelicolor J3411 (pJS94)

Streptomyces coelicolor

697978