3.1.13.3: oligonucleotidase
This is an abbreviated version!
For detailed information about oligonucleotidase, go to the full flat file.
Word Map on EC 3.1.13.3
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3.1.13.3
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exoribonuclease
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exonuclease
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trna
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oligoribonucleotides
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pnpase
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cyclic-di-gmp
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exonucleolytic
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phosphoesterase
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c-di-gmp
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deddh
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3'-phosphoadenosine
- 3.1.13.3
- exoribonuclease
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exonuclease
- trna
- oligoribonucleotides
- pnpase
- cyclic-di-gmp
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exonucleolytic
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phosphoesterase
- c-di-gmp
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deddh
- 3'-phosphoadenosine
Reaction
exonucleolytic cleavage of oligonucleotides to yield nucleoside 5'-phosphates =
Synonyms
CpsORN, EC 3.1.4.19, More, nanoRNase, NrnC, nucleotidase, oligo-, oligoribonuclease, ORN, phosphodiesterase, REXO2, RNase T, small fragment nuclease, XC847, YtqI
ECTree
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Engineering
Engineering on EC 3.1.13.3 - oligonucleotidase
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D163A
site-directed mutagenesis, mutation of the catalytic residue, inactive mutant
H158A
site-directed mutagenesis, the mutant completely loses its hydrolytic cleavage ability
H66A
site-directed mutagenesis, mutation is located on the alpha2 helix, inactive mutant
S108A
site-directed mutagenesis, the mutant exhibits similar activity as the wild-type
S167A
site-directed mutagenesis, mutant exhibits similar activity as the wild-type
Y129A
site-directed mutagenesis, mutant exhibits similar activity as the wild-type
Y129F
site-directed mutagenesis, mutant exhibits similar activity as the wild-type
H158A
Colwellia psychrerythraea ATCC BAA-681
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site-directed mutagenesis, the mutant completely loses its hydrolytic cleavage ability
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S108A
Colwellia psychrerythraea ATCC BAA-681
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site-directed mutagenesis, the mutant exhibits similar activity as the wild-type
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S167A
Colwellia psychrerythraea ATCC BAA-681
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site-directed mutagenesis, mutant exhibits similar activity as the wild-type
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Y129A
Colwellia psychrerythraea ATCC BAA-681
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site-directed mutagenesis, mutant exhibits similar activity as the wild-type
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Y129F
Colwellia psychrerythraea ATCC BAA-681
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site-directed mutagenesis, mutant exhibits similar activity as the wild-type
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D130A
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pJS94, encoding the catalytically inactive point mutant OrnA D130A does not suppress the phenotype of the Streptomyces coelicolor J3411 (DELTAornA::apr) null mutant
additional information
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ytqI mutant, shows impairment of growth in the absence of cysteine
additional information
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ORN mutant, shows growth defect, YtqI from Bacillus subtilis is able to complement the mutant
additional information
construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
additional information
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construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
additional information
generation of enzyme mutant DELTAorn, mRNA levels of pyocin biosynthesis genes in the DELTAorn mutant compared to those in wild-type strain PA14. Mutation of orn drastically increases bacterial susceptibility to quinolones but not to tetracycline, aminoglycoside, or beta-lactam antibiotics. Upregulation of pyocin genes contributes to increased susceptibility to ciprofloxacin in the orn mutant. PrtR stability is reduced by the mutation of orn
additional information
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generation of enzyme mutant DELTAorn, mRNA levels of pyocin biosynthesis genes in the DELTAorn mutant compared to those in wild-type strain PA14. Mutation of orn drastically increases bacterial susceptibility to quinolones but not to tetracycline, aminoglycoside, or beta-lactam antibiotics. Upregulation of pyocin genes contributes to increased susceptibility to ciprofloxacin in the orn mutant. PrtR stability is reduced by the mutation of orn
additional information
generation of enzyme mutant DELTAorn. The lysates from DELTAorn show 25fold decrease in 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) hydrolysis. Complementation with wild-type, but not active site mutants, restores hydrolysis. Accumulation of pGpG in the DELTAorn strain inhibits PDE-As, increasing cyclic-diguanylate (c-di-GMP) concentration. Increased transcription from the cyclic-diguanylate-regulated pel promoter is observed. Additionally, the cyclic-diguanylate-governed auto-aggregation and biofilm phenotypes are elevated in the DELTAorn strain in a pel-dependent manner. Detection of elevated levels of pGpG and cyclic-diguanylate in the DELTAorn strain
additional information
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generation of enzyme mutant DELTAorn. The lysates from DELTAorn show 25fold decrease in 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) hydrolysis. Complementation with wild-type, but not active site mutants, restores hydrolysis. Accumulation of pGpG in the DELTAorn strain inhibits PDE-As, increasing cyclic-diguanylate (c-di-GMP) concentration. Increased transcription from the cyclic-diguanylate-regulated pel promoter is observed. Additionally, the cyclic-diguanylate-governed auto-aggregation and biofilm phenotypes are elevated in the DELTAorn strain in a pel-dependent manner. Detection of elevated levels of pGpG and cyclic-diguanylate in the DELTAorn strain
additional information
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construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
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additional information
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construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
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additional information
-
construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
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additional information
-
construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
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additional information
-
construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
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additional information
-
construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
-
additional information
-
construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels
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additional information
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oligoribonuclease null mutant J3411 (DELTAornA::apr), reveals a conditional defect in morphological differentiation that is reminiscent of bldA. PpJS93, containing ornA under the control of a constitutive promoter, completely suppresses the phenotype of Streptomyces coelicolor J3411 (DELTAornA::apr), but pJS94 (encoding catalytically inactive OrnA D130A) does not