Application | Comment | Organism |
---|---|---|
additional information | YtqI, in addition to oligoribonuclease activity, has 3'-phosphoadenosine 5'-phosphate-phosphatase activity in vitro. YtqI is able to complement both orn and cysQ mutants in Escherichia coli | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression of his-tagged YtqI under control of the arabinose-inducible promoter Para | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ORN mutant, shows growth defect, YtqI from Bacillus subtilis is able to complement the mutant | Escherichia coli |
additional information | ytqI mutant, shows impairment of growth in the absence of cysteine | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-phosphoadenosine 5'-phosphate | inhibits YtqI-catalyzed degradation of nanoRNA. At 0.1, 0.2 or 0.5 mM, the activity of YtqI based on the conversion of 5-mer into monomers in 30 min dropps to 28, 4 and 1%, respectively | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine 5'-phosphate + H2O | - |
Bacillus subtilis | AMP + phosphate | - |
? | |
RNA + H2O | degrades RNA oligonucleotides with preference for 3-mers | Bacillus subtilis | nucleoside 5'-phosphate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ORN | - |
Escherichia coli |
YtqI | - |
Bacillus subtilis |