3.1.11.6: exodeoxyribonuclease VII
This is an abbreviated version!
For detailed information about exodeoxyribonuclease VII, go to the full flat file.
Word Map on EC 3.1.11.6
Reaction
Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates =
Synonyms
E. coli exonuclease VII, endodeoxyribonuclease VII, Escherichia coli exonuclease VII, exonuclease VII, ExoVII, nuclease, exodeoxyribo-, VII, RecBCD, XseA, XseB
ECTree
3.1.11.6 exodeoxyribonuclease VIIAdvanced search results
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Engineering on EC 3.1.11.6 - exodeoxyribonuclease VII
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A188T
site-directed mutagenesis of a highly conserved residue. The A188T mutant shows a msDNA cleavage indistinguishable from that of the wild-type, and lethality is reduced. About 10times more colonies are observed from the A188T mutant than from the wild-type
D155N
site-directed mutagenesis of a highly conserved residue. The D155N mutant loses the ability to cleave msDNA and displays little lethality
DELTA397-456
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deletion mutant of large subunit XseA, complete loss of catalytic activity
G237R
site-directed mutagenesis of a residue in the glycine-rich motif. No msDNA cleavage is found for the G237R mutant but a low level of cell killing is still observed. The culture viability drops to about 30% after a 2 h induction
R64E/R68E/R69E
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mutant of large subunit XseA, decrease in DNA-binding activity
additional information
additional information
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construction of recD mutants showing a RecBC enzyme, instead of a RecBCD enzyme, lacking the RecD subunit and exonuclease VII activity, the mutation affects phage lambda red gam recombination, but does not cause an altered phenotype, overview
additional information
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mutant in all four exonucleases (ExoI, ExoVII, ExoX, and RecJ) exhibits pronounced 20fold elevation of cross-overs between 25 bp of homology relative to the wild-type
additional information
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recJ xseA sbcCD xonA2 quadruple mutants are cold sensitive depending on the xon allele. Absence of the two 3' exonucleases ExoI and ExoVII allows highly efficient conjugational recombination in recBCD+ cells, which is independent of SbcCD, recJ xseA double mutant has lower UV-survival than wild-type
additional information
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conbstruction of ExoVII-deficient cells and of cells lacking alll three exonuxleases, exonuclease I, exonuclease VII, or SbcCD, phenotypes, detailed overview
additional information
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construction of a series of subunit XseA deletion variants that lack one or several of the helices. Variants with a single helix deleted bind about 70% and variants with two helices deleted about 50% of the subunit XseB levels compared to the full-length XseA. All these deletion variants exhibit a complete loss of exonucleolytic activity in vitro
additional information
construction of ExoVII- (xseB) and ExoVII-(xseA) deficient mutant strains which are both inactive with msDNA. Construction of promoter deletion mutants, i.e. by deletion of entire promoter (large deletion from -125 to -11) or promoter-element mutation (-36T->C). Overexpression of the large subunit XseA is lethal, but the small subunit counteracts the toxicity of the large subunit. In samples containing both pCC-XseA and pT-XseB, overproduction of XseA by induction has little effect on the viability. RecA also protects cells from death by XseA overexpression. MsDNA cleavage and cell death at various subunit ratios, overview
additional information
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construction of ExoVII- (xseB) and ExoVII-(xseA) deficient mutant strains which are both inactive with msDNA. Construction of promoter deletion mutants, i.e. by deletion of entire promoter (large deletion from -125 to -11) or promoter-element mutation (-36T->C). Overexpression of the large subunit XseA is lethal, but the small subunit counteracts the toxicity of the large subunit. In samples containing both pCC-XseA and pT-XseB, overproduction of XseA by induction has little effect on the viability. RecA also protects cells from death by XseA overexpression. MsDNA cleavage and cell death at various subunit ratios, overview
additional information
effect of xonA (ExoI), xseA (ExoVII) and sbcCD (SbcCD) mutations on growth without DnaA (dnaA46 at 42°C). The replication fork trap in the termination area is inactivated by deletion of tus and an rpoB*35 point mutation is introduced to alleviate replication-transcription conflicts resulting from replication forks travelling in a direction opposite to normal. The DnaA-independent growth is triggered by the absence of 3' exonucleases. Generation of RCe267 (dnaA46 DELTAtus rpo*), RCe528 (dnaA46 DELTAtus rpo* DELTAxonA), SLM1219 (dnaA46 DELTAtus rpo* DELTAxseA), RCe553 (dnaA46 DELTAtus rpo* DELTAsbcCD), SLM1194 (dnaA46 DELTAtus rpo* DELTAxonA* DELTAxseA), RCe554 (dnaA46 DELTAtus rpo* DELTAxonA DELTAsbcCD), SLM1223 (dnaA46 DELTAtus rpo* DELTAxseA DELTAsbcCD) and SLM1226 (dnaA46 DELTAtus rpo* DELTAxonA DELTAxseA DELTAsbcCD) mutant strains, phenotypes, overview. Overreplication of the termination area in cells lacking 3' exonucleases is abolished if the chromosome is linearized. And overreplication of the termination area in cells lacking 3' exonucleases requires PriA helicase activity. An asymmetric replichore arrangement does not increase overreplication of the termination area in cells lacking 3' exonucleases
additional information
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effect of xonA (ExoI), xseA (ExoVII) and sbcCD (SbcCD) mutations on growth without DnaA (dnaA46 at 42°C). The replication fork trap in the termination area is inactivated by deletion of tus and an rpoB*35 point mutation is introduced to alleviate replication-transcription conflicts resulting from replication forks travelling in a direction opposite to normal. The DnaA-independent growth is triggered by the absence of 3' exonucleases. Generation of RCe267 (dnaA46 DELTAtus rpo*), RCe528 (dnaA46 DELTAtus rpo* DELTAxonA), SLM1219 (dnaA46 DELTAtus rpo* DELTAxseA), RCe553 (dnaA46 DELTAtus rpo* DELTAsbcCD), SLM1194 (dnaA46 DELTAtus rpo* DELTAxonA* DELTAxseA), RCe554 (dnaA46 DELTAtus rpo* DELTAxonA DELTAsbcCD), SLM1223 (dnaA46 DELTAtus rpo* DELTAxseA DELTAsbcCD) and SLM1226 (dnaA46 DELTAtus rpo* DELTAxonA DELTAxseA DELTAsbcCD) mutant strains, phenotypes, overview. Overreplication of the termination area in cells lacking 3' exonucleases is abolished if the chromosome is linearized. And overreplication of the termination area in cells lacking 3' exonucleases requires PriA helicase activity. An asymmetric replichore arrangement does not increase overreplication of the termination area in cells lacking 3' exonucleases
additional information
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mutant in all four exonucleases (ExoI, ExoVII, ExoX, and RecJ) exhibits pronounced 20fold elevation of cross-overs between 25 bp of homology relative to the wild-type
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additional information
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conbstruction of ExoVII-deficient cells and of cells lacking alll three exonuxleases, exonuclease I, exonuclease VII, or SbcCD, phenotypes, detailed overview
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