1.1.1.9: D-xylulose reductase

This is an abbreviated version, for detailed information about D-xylulose reductase, go to the full flat file.

Reaction

xylitol
+
NAD+
=
D-xylulose
+
NADH
+
H+

Synonyms

2,3-cis-polyol(DPN) dehydrogenase (C3-5), erythritol dehydrogenase, GmXDH, NAD-dependent xylitol dehydrogenase, pentitol-DPN dehydrogenase, PsXDH, reductase, D-xylulose, slSDH, XDH, XDH-Y25, xdhA, XL2, XYL2, xylitol dehydrogenase, xylitol-2-dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.9 D-xylulose reductase

Temperature Stability

Temperature Stability on EC 1.1.1.9 - D-xylulose reductase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 40
-
10 min, 2.5 mM NAD+, stable
20
-
at room temperature, the enzyme is inactivated within 2 days
25
-
and below stable
30
-
1 h, completely stable
30 - 45
-
the enzyme exhibits activities of approximately 81% and 51% after being incubated at 30°C and 35°C, respectively, for 30 min. The enzyme completely loses its activity at temperatures greater than 45°C
40
half-life 120 min
45 - 50
approximately 92% and 70% activities remain following incubations at 45 and 50°C, respectively. The enzyme completely loses its activity above 60°C
53.1
half denaturation temperature
additional information
-
wild-type: half denaturation temperature T1/2 (°C): 35.2, thermal transition temperature Tcd (°C): 43.0