1.1.1.9: D-xylulose reductase

This is an abbreviated version, for detailed information about D-xylulose reductase, go to the full flat file.

Reaction

xylitol
+
NAD+
=
D-xylulose
+
NADH
+
H+

Synonyms

2,3-cis-polyol(DPN) dehydrogenase (C3-5), erythritol dehydrogenase, GmXDH, NAD-dependent xylitol dehydrogenase, pentitol-DPN dehydrogenase, PsXDH, reductase, D-xylulose, slSDH, XDH, XDH-Y25, xdhA, XL2, XYL2, xylitol dehydrogenase, xylitol-2-dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.9 D-xylulose reductase

Cloned

Cloned on EC 1.1.1.9 - D-xylulose reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein in Escherichia coli
-
expressed in Candida glycerinogenes
-
expressed in Candida glycerinogenes strain WL2002-5
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM 109
-
expressed in Saccharomyces cerevisiae strain DX23
-
expressed in transketolase-deficient Saccharomyces cerevisiae strain (W303/tkl1 tkl2/2C)
-
expression in Escherichia coli
expression of the engineered D202A/L203R/V204S/E205P/S206R mutant enzyme fron Galactocandida mastotermitis with altered cofactor specificity, co-expression with a mutant NADPH-specific xylulose reductase from Candida tenuis in Saccharomyces cerevisiae, the transformed strain shows up to 50% decreased glycerol yield without increase in ethanol during xylose fermentation, overview
-
gene xdhA, coexpression of gene xdhA and cofactor regeneration enzyme, i.e. glucose dehydrogenase gene gdh from Bacillus subtilis, in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
-
genes XYL2 (D207A/I208R/F209S) and XYL2 (S96C/S99C/Y102C/D207A/I208R/F209S) are introduced into Saccharomyces cerevisiae, which already contain the Pichia stipitis XYL1 gene (encoding xylose reductase) and the endogenously overexpressed XKS1 gene (encoding xylulokinase)
-
overexpression in Saccharomyces cerevisiae
-
overexpression of the enzyme in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, co-expression with xylose reductase from Candida tenuis
-