Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhizobium etli |
Crystallization (Comment) | Organism |
---|---|
multi-template homology modeling. The structural model of XDH obtained consists of a classical alpha/beta Rossmann fold pattern commonly found in the MDR family, which is organized into two beta-barrel domains, the coenzyme-binding residues 163-300 and catalytic residues 1-162, 301-364 | Rhizobium etli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1-10 mM, strong inhibition | Rhizobium etli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
NAD+ | pH 9.5, 25°C | Rhizobium etli | |
12.1 | - |
xylitol | pH 9.5, 25°C | Rhizobium etli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 18% of the activity with Mg2+ | Rhizobium etli | |
Co2+ | 1 mM, 38% of the activity with Mg2+ | Rhizobium etli | |
Cu2+ | 1 mM, 51% of the activity with Mg2+ | Rhizobium etli | |
Mg2+ | or Mn2+, required | Rhizobium etli | |
Mn2+ | or Mg2+, required. At 1 mM, 108% of the activity with Mg2+ | Rhizobium etli | |
additional information | activity is not significantly stimulated by Ba2+, Ca2+, Fe2+, Hg2+, or K+ | Rhizobium etli | |
Zn2+ | 1 mM, 62% of the activity with Mg2+. The deduced XDH gene product possesses the Zn2+-containing ADH signature sequence [GHE]xx[G]xxxxx[G]xx[V] and the catalytic Zn2+-binding site Cys41, His66, Glu67, and Glu152 | Rhizobium etli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
4 * 35858, calculated, 4 * 34000, SDS-PAGE | Rhizobium etli |
35858 | - |
4 * 35858, calculated, 4 * 34000, SDS-PAGE | Rhizobium etli |
135000 | - |
gel filtration | Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | Q2K0Q7 | strain CFN42 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzmye | Rhizobium etli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD+ | 8% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
D-sorbitol + NAD+ | 90% of the activity with xylitol | Rhizobium etli | D-fructose + NADH + H+ | - |
? | |
erythritol + NAD+ | 0.77% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
galactitol + NAD+ | 0.98% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
L-arabinitol + NAD+ | 21% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
L-threitol + NAD+ | 0.05% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
L-threitol + NAD+ | 75% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
ribitol + NAD+ | 40% of the activity with xylitol | Rhizobium etli | ? + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Rhizobium etli | D-xylulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35858, calculated, 4 * 34000, SDS-PAGE | Rhizobium etli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Rhizobium etli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life 120 min | Rhizobium etli |
53.1 | - |
half denaturation temperature | Rhizobium etli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Rhizobium etli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | activity with NADP+ is 4% of the activity with NAD+ | Rhizobium etli | |
NAD+ | the deduced XDH gene product possesses an NAD-binding glycine-rich Rossmann fold domain [GXGXXG] present in MDR superfamily | Rhizobium etli |