EC Number   |
Protein Variants |
Reference |
|---|
   6.3.2.30 | C595S |
enhanced catalytic activity |
-, 713897 |
| 6.3.2.30 | C595S |
site-directed mutagenesis, the mutant shows a 2.5fold increased catalytic activity compared to the wild-type enzyme |
701820 |
| 6.3.2.30 | E856A |
site-directed mutagenesis, inactive mutant |
-, 691115 |
| 6.3.2.30 | E856V |
site-directed mutagenesis, inactive mutant |
-, 691115 |
| 6.3.2.30 | more |
construction of deletion mutants CphA6308DELTA1, CphA6308DELTA2, and CphA6308DELTA3, which are truncated by one, two, or three amino acid(s), respectively, at the C-terminus, and a combination of the deletion mutation with point mutation C595S, mutants CphA6308DELTA1 and CphA6308DELTA1/C595S show increased catalytic activity compared to the wild-type enzyme, while mutants CphA6308DELTA2 and CphA6308DELTA3 show similar or reduced CphA enzyme activity in comparison to wild-type CphA6308 |
701820 |
| 6.3.2.30 | more |
construction of truncation mutant CphANE1-C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1-C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability |
-, 691115 |
| 6.3.2.30 | more |
generation of transgenic Solanum tuberosum plants that recombinantly produce the bacterial enzyme by tuber-specific expression. The mutant plants show increased cyanophycin contents, and the tuber-specific expression minimizes the phenotypic abnormalities of the sprout caused by cyanophycin accumulation, phenotypes,overview |
706150 |
| 6.3.2.30 | more |
two truncated CphAs, lacking 31 (CphANE1del96) or 59 (CphANE1del180) amino acids of the C-terminal region, are derived from cphANE1 by deleting 96 or 180 bp from its 3' region through the introduction of stop codons. In comparison to the wild-type gene, cphANE1del96 conferrs about 2.1-2.2fold higher enzyme activity on Escherichia coli. These cells accumulate about twofold more cyanophycin than cells expressing the wild-type gene |
671478 |
