Cloned (Comment) | Organism |
---|---|
engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli | Nostoc ellipsosporum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | two truncated CphAs, lacking 31 (CphANE1del96) or 59 (CphANE1del180) amino acids of the C-terminal region, are derived from cphANE1 by deleting 96 or 180 bp from its 3' region through the introduction of stop codons. In comparison to the wild-type gene, cphANE1del96 conferrs about 2.1-2.2fold higher enzyme activity on Escherichia coli. These cells accumulate about twofold more cyanophycin than cells expressing the wild-type gene | Nostoc ellipsosporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc ellipsosporum | Q0H8A5 | transposon-induced argL mutant NE1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | L-Lys cannot replace L-Arg in reaction with CphANE1 or CphANE1del96 | Nostoc ellipsosporum | [L-Asp(4-L-Arg)]n+1 + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CphANE1 | - |
Nostoc ellipsosporum |