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Literature summary for 6.3.2.30 extracted from

  • Hai, T.; Frey, K.M.; Steinbuechel, A.
    Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli (2006), Appl. Environ. Microbiol., 72, 7652-7660.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli Nostoc ellipsosporum

Protein Variants

Protein Variants Comment Organism
additional information two truncated CphAs, lacking 31 (CphANE1del96) or 59 (CphANE1del180) amino acids of the C-terminal region, are derived from cphANE1 by deleting 96 or 180 bp from its 3' region through the introduction of stop codons. In comparison to the wild-type gene, cphANE1del96 conferrs about 2.1-2.2fold higher enzyme activity on Escherichia coli. These cells accumulate about twofold more cyanophycin than cells expressing the wild-type gene Nostoc ellipsosporum

Organism

Organism UniProt Comment Textmining
Nostoc ellipsosporum Q0H8A5 transposon-induced argL mutant NE1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg L-Lys cannot replace L-Arg in reaction with CphANE1 or CphANE1del96 Nostoc ellipsosporum [L-Asp(4-L-Arg)]n+1 + ADP + phosphate
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Synonyms

Synonyms Comment Organism
CphANE1
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Nostoc ellipsosporum