Literature summary for 6.3.2.30 extracted from

Huehns, M.; Neumann, K.; Hausmann, T.; Klemke, F.; Lockau, W.; Kahmann, U.; Kopertekh, L.; Staiger, D.; Pistorius, E.K.; Reuther, J.; Waldvogel, E.; Wohlleben, W.; Effmert, M.; Junghans, H.; Neubauer, K.; Kragl, U.; Schmidt, K.; Schmidtke, J.; Broer, I.
Tuber-specific cphA expression to enhance cyanophycin production in potatoes (2009), Plant Biotechnol. J., 7, 883-898.

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Application

Application	Comment	Organism
synthesis	production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products by the recombinant enzyme in transgenic plants	Thermosynechococcus vestitus
synthesis	restriction of cyanophycin accumulation to the potato tubers by using the cyanophycin synthetase gene from Thermosynechococcus elongatus BP-1, under the control of the tuber-specific class 1 promoter. Tuber-specific cytosolic expression by pB33-cphATe as well as tuber-specific plastidic expression by pB33-PsbYcphATe results in significant polymer accumulation solely in the tubers. In plants transformed with pB33-cphATe, both cyanophycin synthetase and cyanophycin are detected in the cytoplasm leading to an increase up to 2.3% cyanophycin of dry weight and resulting in small and deformed tubers. In B33-PsbY-cphATe tubers, cyanophycin synthetase and cyanophycin are exclusively found in amyloplasts leading to a cyanophycin accumulation up to 7.5% of dry weight. These tubers are normal in size, some clones show reduced tuber yield and sometimes exhibit brown sunken staining starting at tubers navel. During a storage period over of 32 weeks of one selected clone, the cyanophycin content was stable in B33-PsbYcphATe tubers but the stress symptoms increased. Nitrogen fertilization in the greenhouse does not lead not to an increased cyanophycin yield, slightly reduced protein content, decreased starch content, and changes in the amounts of bound and free arginine and aspartate	Thermosynechococcus vestitus

Cloned(Commentary)

Cloned (Comment)	Organism
gene cphA, expression in Solanum tuberosum tubers under control of the tuber-specific class 1 promoter B33 directed to tuber cytosol or to tuber plastids leading to cyanophycin accumulation in the tubers	Thermosynechococcus vestitus

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of transgenic Solanum tuberosum plants that recombinantly produce the bacterial enzyme by tuber-specific expression. The mutant plants show increased cyanophycin contents, and the tuber-specific expression minimizes the phenotypic abnormalities of the sprout caused by cyanophycin accumulation, phenotypes,overview	Thermosynechococcus vestitus

Organism

Organism	UniProt	Comment	Textmining
Thermosynechococcus vestitus	-	-	-
Thermosynechococcus vestitus	-	gene cphA	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	potato tuber-specific expression of gene cphA	Thermosynechococcus vestitus	-

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Release 2023.1 (January 2023)