EC Number   |
Protein Variants |
Reference |
|---|
   6.2.1.64 | DELTA946 |
truncation of the Uba1 carboxyl-terminal beta-grasp domain reduces cognate Ubc2b binding by 31-fold and kcat by 33500-fold |
727923 |
| 6.2.1.64 | DELTA946 |
truncation of the Uba3 carboxyl-terminal beta-grasp domain, no effect on cognate Ubc12 thiolester formation |
727923 |
| 6.2.1.64 | I310N |
the mutations in the catalytic subunit UBA3 increases the enzyme's affinity for ATP while decreasing its affinity for NEDD8. This mutation effectively contributes to decreased MLN4924 potency in vitro while providing for sufficient enzyme function for leukemia cell survival |
758191 |
| 6.2.1.64 | more |
generation of a chimeric Ub1 isoform carrying the beta-grasp domain of Uba3. Ternary complex stoichiometries for wild type and chimeric Uba1 are identical showing that the beta-grasp domain is not required in the Uba1 catalytic cycle |
727923 |
| 6.2.1.64 | Y352H |
the mutations in the catalytic subunit UBA3 increases the enzyme's affinity for ATP while decreasing its affinity for NEDD8. This mutation effectively contributes to decreased MLN4924 potency in vitro while providing for sufficient enzyme function for leukemia cell survival |
758191 |
