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Information on EC 6.2.1.64 - E1 NEDD8-activating enzyme

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EC Tree
     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.64 E1 NEDD8-activating enzyme
IUBMB Comments
Some RING-type E3 ubiquitin transferase (EC 2.3.2.27) are not able to bind a substrate protein directly. Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING-Ligase) complexes. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. Like ubiquitin, the NEDD8 protein ends with two glycine residues. The E1 NEDD8-activating enzyme activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues. The activated NEDD8 is subsequently transferred to a cysteine residue of EC 2.3.2.34, E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC 2.3.2.32, cullin-RING-type E3 NEDD8 transferase).
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Word Map
  • 6.2.1.64
  • neddylation
  • ligases
  • cullin-ring
  • cullins
  • ubiquitin-like
  • pevonedistat
  • mln4924-induced
  • first-in-class
  • cdt1
  • rereplication
  • nedd8-conjugating
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
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[NEDD8 protein]
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[E1 NEDD8-activating enzyme]-L-cysteine
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[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Synonyms
activating enzyme for NEDD8, activating enzyme of NEDD8, APP-BP-1, APP-BP1, APPBP1, APPBP1-UBA3, APPBP1-UBA3 complex, APPBP1/UBA3, E1 enzyme for NEDD8, E1 NEDD8, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
show the reaction diagram
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PATHWAY SOURCE
PATHWAYS
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