show all | hide all No of entries

Information on EC - E1 NEDD8-activating enzyme

for references in articles please use BRENDA:EC6.2.1.64
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
       E1 NEDD8-activating enzyme
IUBMB Comments
Some RING-type E3 ubiquitin transferase (EC are not able to bind a substrate protein directly. Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING-Ligase) complexes. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. Like ubiquitin, the NEDD8 protein ends with two glycine residues. The E1 NEDD8-activating enzyme activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues. The activated NEDD8 is subsequently transferred to a cysteine residue of EC, E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC, cullin-RING-type E3 NEDD8 transferase).
Specify your search results
Select one or more organisms in this record: ?
Word Map
  • neddylation
  • ligases
  • cullin-ring
  • cullins
  • ubiquitin-like
  • pevonedistat
  • mln4924-induced
  • first-in-class
  • cdt1
  • rereplication
  • nedd8-conjugating
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
[NEDD8 protein]
[E1 NEDD8-activating enzyme]-L-cysteine
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
activating enzyme for NEDD8, activating enzyme of NEDD8, APP-BP-1, APP-BP1, APPBP1, APPBP1-UBA3, APPBP1-UBA3 complex, APPBP1/UBA3, E1 enzyme for NEDD8, E1 NEDD8, more
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
show the reaction diagram
Select items on the left to see more content.