Literature summary for 6.2.1.64 extracted from

Tokgoez, Z.; Siepmann, T.J.; Streich, F.; Kumar, B.; Klein, J.M.; Haas, A.L.
E1-E2 interactions in ubiquitin and Nedd8 ligation pathways (2012), J. Biol. Chem., 287, 311-321.

Search for more literature for 6.2.1.64

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA946	truncation of the Uba1 carboxyl-terminal beta-grasp domain reduces cognate Ubc2b binding by 31-fold and kcat by 33500-fold	Homo sapiens
DELTA946	truncation of the Uba3 carboxyl-terminal beta-grasp domain, no effect on cognate Ubc12 thiolester formation	Homo sapiens
additional information	generation of a chimeric Ub1 isoform carrying the beta-grasp domain of Uba3. Ternary complex stoichiometries for wild type and chimeric Uba1 are identical showing that the beta-grasp domain is not required in the Uba1 catalytic cycle	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0006	-	ubiquitin	mutant DELTA946, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.0008	-	ubiquitin	wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.0012	-	ubiquitin	chimeric Ub1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.0045	-	ATP	chimeric Ub1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.0054	-	ATP	mutant DELTA946, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.0055	-	ATP	wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.02	-	ATP	mutant DELTA348, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.103	-	ATP	wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P22314	-	-
Homo sapiens	Q8TBC4	isoform Uba3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + ubiquitin + [ubiquitin-activating protein UBA1]-L-cysteine	-	Homo sapiens	AMP + diphosphate + [ubiquitin-activating protein UBA1]-S-ubiquitinyl-L-cysteine	-	?
ATP + ubiquitin + [ubiquitin-activating protein Uba3]-L-cysteine	-	Homo sapiens	AMP + diphosphate + [ubiquitin-activating protein Uba3]-S-ubiquitinyl-L-cysteine	-	?

Synonyms

Synonyms	Comment	Organism
NEDD8-activating enzyme E1 catalytic subunit	-	Homo sapiens
Uba1	-	Homo sapiens
Uba3	-	Homo sapiens
ubiquitin-like modifier-activating enzyme 1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000075	-	ATP	chimeric Ub1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
0.00013	-	ATP	mutant DELTA946, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
3.1	-	ATP	mutant DELTA348, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
3.4	-	ATP	wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
3.5	-	ATP	wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)