EC Number   |
Protein Variants |
Reference  |
|---|
    6.2.1.3 | N431A |
18 site-directed mutantions within the fatty acyl-CoA synthetase structural gene fadD corresponding to the FACS signature. Only one substitution results in wild-type fatty acyl-CoA synthetase activity profiles, 10 substitutions with abolished or greatly diminished enzyme activity, and seven substitutions with altered fatty acid chain length specificity. Of these seven, five also have reduced activity compared with the wild-type enzyme |
739 |
| 6.2.1.3 | E361A |
inactive mutant enzyme |
652285 |
| 6.2.1.3 | G216A |
the ratio of turnover number to Km-value for the reaction with oleate is 32.7% of the wild-type ratio |
652285 |
| 6.2.1.3 | G219A |
the ratio of turnover number to Km-value for the reaction with oleate is 1.3fold higher than the wild-type ratio |
652285 |
| 6.2.1.3 | K222A |
the ratio of turnover number to Km-value for the reaction with oleate is 8% of the wild-type ratio |
652285 |
| 6.2.1.3 | T214A |
the ratio of turnover number to Km-value for the reaction with oleate is 10.3% of the wild-type ratio |
652285 |
| 6.2.1.3 | T217A |
the ratio of turnover number to Km-value for the reaction with oleate is 6.7% of the wild-type ratio |
652285 |
| 6.2.1.3 | Y213A |
inactive mutant enzyme |
652285 |
| 6.2.1.3 | G401L |
inactive enzyme |
672417 |
| 6.2.1.3 | L399M |
no significant alteration of activity compared to the wild type enzyme |
672417 |
